Skeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat meal

Molecular Metabolism
Michael D TarpeyMatthew W Hulver

Abstract

We tested the hypothesis that skeletal muscle of endurance-trained male runners would exhibit elevated autophagy and mitophagy markers, which would be associated with greater metabolic flexibility following a high-fat meal (HFM). Muscle biopsies were collected to determine differences in autophagy and mitophagy protein markers and metabolic flexibility under fasting conditions and 4 h following a HFM between endurance-trained male runners (n = 10) and sedentary, non-obese controls (n = 9). Maximal oxygen consumption (ml·kg·min-1) was approximately 50% higher (p < 0.05) in endurance-trained runners compared with sedentary controls (65.8 ± 2.3 and 43.1 ± 3.4, respectively). Autophagy markers were similar between groups. Mitophagy and mitochondrial dynamics protein markers were significantly higher in skeletal muscle of endurance-trained runners compared with sedentary controls in the fasted state, although unaffected by the HFM. Skeletal muscle metabolic flexibility was similar between groups when fasted (p > 0.05), but increased in response to the HFM in endurance-trained athletes only (p < 0.005). Key mitophagy markers, phospho-Pink1Thr257 and phospho-ParkinS65 (r = 0.64, p < 0.005), and phospo-ParkinSer65 and phospho-Drp1Ser61...Continue Reading

Citations

Sep 15, 2018·Annual Review of Physiology·David A HoodMatthew Triolo
Aug 29, 2020·Journal of Cachexia, Sarcopenia and Muscle·Sanaz MehrabaniAmirhossein Sahebkar
Mar 13, 2020·Journal of Applied Physiology·Mary Elizabeth BaughKevin P Davy
Sep 27, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lidan ZhaoRobert P Rhoads
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Methods Mentioned

BETA
biopsies
biopsy
ubiquitination

Software Mentioned

Image Lab

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