DOI: 10.1101/494559Dec 13, 2018Paper

Skeletal muscle ceramides do not contribute to physical inactivity-induced insulin resistance

BioRxiv : the Preprint Server for Biology
Zéphyra AppriouFrédéric Derbré

Abstract

Physical inactivity increases the risk to develop type 2 diabetes, a disease characterized by a state of insulin resistance. By promoting inflammatory state, ceramides are especially recognized to alter insulin sensitivity in skeletal muscle. The present study was designed to analyze, in mice, whether muscle ceramides contribute to physical inactivity-induced insulin resistance. For this purpose, we used the wheel lock model to induce a sudden reduction of physical activity, in combination with myriocin treatment, an inhibitor of de novo ceramide synthesis. Mice were assigned to 3 experimental groups: voluntary wheel access group (Active), a wheel lock group (Inactive) and wheel lock group treated with myriocin (Inactive-Myr). We observed that 10 days of physical inactivity induces hyperinsulinemia and increase HOMA-IR. The muscle ceramide content were not modified by physical inactivity and myriocin. Thus, muscle ceramides do not play a role in physical inactivity-induced insulin resistance. In skeletal muscle, insulin-stimulated Akt phosphorylation and inflammatory pathway were not affected by physical inactivity whereas a reduction of GLUT4 content was observed. Based on these results, physical inactivity-induced insulin res...Continue Reading

Related Concepts

Ceramides
Diabetes Mellitus, Non-Insulin-Dependent
Hyperinsulinism
Insulin
Insulin Resistance
Laboratory mice
Muscle
Phosphorylation
Thermozymocidin
Glucose Transporter Type 4

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