Skeletal muscle fiber-type-specific changes in markers of capillary and mitochondrial content after low-volume interval training in overweight women

Physiological Reports
Rachel TanMartin J Gibala

Abstract

High-intensity interval training (HIIT) enhances skeletal muscle oxygen delivery and utilization but data are limited regarding fiber-specific adaptations in humans. We examined the effect of 18 sessions of HIIT (10 × 60-sec cycling intervals at ~90% HRmax , interspersed by 60-sec of recovery) over 6 weeks on markers of microvascular density and oxidative capacity in type I and II fibers in healthy but sedentary young women (Age: 26 ± 7 years; BMI: 30 ± 4 kg·m-2 ; VO2peak : 2.16 ± 0.45 L·m-1 ). Immunohistochemical analyses of muscle cross sections revealed a training-induced increase in capillary contacts per fiber in type I fibers (PRE: 4.38 ± 0.37 vs. POST: 5.17 ± 0.80; main effect, P < 0.05) and type II fibers (PRE: 4.24 ± 0.55 vs. POST: 4.92 ± 0.54; main effect, P < 0.05). The capillary-to-fiber ratio also increased after training in type I fibers (PRE: 1.53 ± 1.44 vs. POST: 1.88 ± 0.38; main effect, P < 0.05) and type II fibers (PRE: 1.45 ± 0.19 vs. POST: 1.76 ± 0.27; main effect, P < 0.05). Muscle oxidative capacity as reflected by the protein content of cytochrome oxidase IV also increased after training in type I fibers (PRE: 3500 ± 858 vs. POST: 4442 ± 1377 arbitrary units; main effect, P < 0.01) and type II fibers (PR...Continue Reading

References

Jul 1, 1976·Acta Physiologica Scandinavica·J Henriksson, J S Reitman
Jan 1, 1997·Pflügers Archiv : European journal of physiology·R T HeppleM J Plyley
Jan 27, 2000·Medicine and Science in Sports and Exercise·D R Bassett, E T Howley
Aug 4, 2004·Pflügers Archiv : European journal of physiology·Peter KrustrupJens Bangsbo
Aug 16, 2008·Pflügers Archiv : European journal of physiology·Stuart Egginton
Jan 24, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Birgitte Hoier, Ylva Hellsten
Mar 7, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rebecca L ScalzoBenjamin F Miller
Apr 29, 2014·European Journal of Sport Science·Joseph T CostelloChris M Bleakley
Feb 3, 2015·The Journal of Physiology·Dorte E KristensenJørgen F P Wojtaszewski
May 31, 2016·Journal of Cachexia, Sarcopenia and Muscle·Joshua P NederveenGianni Parise
Nov 30, 2016·Medicine and Science in Sports and Exercise·Mia A SchaumbergTina L Skinner
Jan 25, 2017·Experimental Physiology·Lauren E SkellyMartin J Gibala

❮ Previous
Next ❯

Citations

Sep 26, 2020·International Journal of Molecular Sciences·Amanda J GendersDavid J Bishop
Aug 14, 2020·American Journal of Physiology. Cell Physiology·Irene S Tobias, Andrew J Galpin
Dec 24, 2021·Sports Medicine - Open·Lauren E SkellyJenna B Gillen

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies

Software Mentioned

SPSS
Power
Nikon NIS Elements AR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.