Skeletal muscle type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression: effects of obesity and endurance training

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Paul J LeBlancSandra J Peters

Abstract

Pyruvate dehydrogenase (PDH) plays an important role in regulating carbohydrate metabolism in skeletal muscle. PDH is activated by PDH phosphatase (PDP) and deactivated by PDH kinase (PDK). Obesity has a large negative impact on skeletal muscle carbohydrate metabolism, whereas endurance training has been shown to improve regulatory control of skeletal muscle carbohydrate metabolism, more so when coupled with obesity. A majority of this literature has focused on PDK, with little information available on PDP. To determine the relative role of PDP in regulating skeletal muscle PDH activity with obesity and endurance training, obese and lean Zucker rats remained sedentary or were endurance trained (1 h/day, 5 days/wk) for a period of 8 wk. Soleus, red gastrocnemius, (RG), and white gastrocnemius (WG) muscles were sampled after the training period. The main findings were 1) obesity resulted in a 46% decrease in PDP activity expressed per milligram extracted mitochondrial protein only in RG, while PDP isoform content was unchanged; 2) 8 wk of endurance training led to a significant 1.4-2.2-fold increase in PDP activity of all muscle examined from obese rats, and the concomitant increase in PDP1 protein was only seen in soleus and RG;...Continue Reading

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Oct 5, 2006·American Journal of Physiology. Endocrinology and Metabolism·Paul J LeblancSandra J Peters

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Citations

Mar 26, 2010·The Journal of Membrane Biology·Leslie E StefanykPaul J LeBlanc
May 6, 2009·Biochimica Et Biophysica Acta·Richard M Denton
Oct 30, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Patrick C TurnbullSandra J Peters
Mar 18, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Emily C DunfordSandra J Peters
May 26, 2016·American Journal of Physiology. Endocrinology and Metabolism·Leslie A ConsittJoseph A Houmard

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