Skeletal myoblast transplantation for repair of myocardial necrosis

The Journal of Clinical Investigation
C E MurryS D Hauschka

Abstract

Myocardial infarcts heal by scarring because myocardium cannot regenerate. To determine if skeletal myoblasts could establish new contractile tissue, hearts of adult inbred rats were injured by freeze-thaw, and 3-4.5 x 10(6) neonatal skeletal muscle cells were transplanted immediately thereafter. At 1 d the graft cells were proliferating and did not express myosin heavy chain (MHC). By 3 d, multinucleated myotubes were present which expressed both embryonic and fast fiber MHCs. At 2 wk, electron microscopy demonstrated possible satellite stem cells. By 7 wk the grafts began expressing beta-MHC, a hallmark of the slow fiber phenotype; coexpression of embryonic, fast, and beta-MHC continued through 3 mo. Transplanting myoblasts 1 wk after injury yielded comparable results, except that grafts expressed beta-MHC sooner (by 2 wk). Grafts never expressed cardiac-specific MHC-alpha. Wounds containing 2-wk-old myoblast grafts contracted when stimulated ex vivo, and high frequency stimulation induced tetanus. Furthermore, the grafts could perform a cardiac-like duty cycle, alternating tetanus and relaxation, for at least 6 min. Thus, skeletal myoblasts can establish new muscle tissue when grafted into injured hearts, and this muscle can...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M J KushmerickR W Wiseman
Aug 1, 1987·The Journal of Cell Biology·C H Clegg, S D Hauschka
Feb 1, 1986·Circulation Research·J D MannionL W Stephenson
Dec 1, 1982·The Journal of Cell Biology·D BaderD A Fischman
Oct 1, 1995·The Journal of Clinical Investigation·G Y KohL J Field
Jul 1, 1995·The Annals of Thoracic Surgery·R C ChiuR L Kao
Mar 27, 1995·Annals of the New York Academy of Sciences·S K TamB Nadal-Ginard
Sep 1, 1993·Neuromuscular Disorders : NMD·C Cifuentes-DiazR M Mège
Sep 1, 1993·The Journal of Clinical Investigation·G Y KohL J Field
Feb 1, 1993·Developmental Biology·S BaloghP A Merrifield
Nov 15, 1996·The Journal of Clinical Investigation·C E MurryS M Schwartz

❮ Previous
Next ❯

Citations

Feb 19, 2000·Microscopy Research and Technique·D Skuk, J P Tremblay
Sep 17, 2002·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Shinji TomitaSoichiro Kitamura
Sep 7, 2006·Cell and Tissue Banking·Oon Cheong OoiReida M El Oakley
Aug 29, 2006·Heart Failure Reviews·E LeontiadisD V Cokkinos
Mar 24, 2006·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Olivier GheysensJoseph C Wu
Jan 27, 2011·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Olivier GheysensSanjiv S Gambhir
Aug 31, 2012·General Thoracic and Cardiovascular Surgery·Hidetoshi Masumoto, Ryuzo Sakata
Nov 8, 2012·General Thoracic and Cardiovascular Surgery·Yoshiki Sawa
Mar 8, 2008·Current Treatment Options in Cardiovascular Medicine·Adam J T SchuldtIra S Cohen
Nov 11, 2010·Current Treatment Options in Cardiovascular Medicine·David A D'Alessandro, Robert E Michler
Jan 8, 2008·Cardiovascular Toxicology·Nikki Gillum, Narine Sarvazyan
Mar 1, 2008·Journal of Cardiovascular Translational Research·Luda KhaitRavi K Birla
Mar 1, 2008·Journal of Cardiovascular Translational Research·Jonathan H Dinsmore, Nabil Dib
Jun 1, 2008·Journal of Cardiovascular Translational Research·Cindy M Martin
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·John K FraserMarc H Hedrick
Oct 15, 2003·Trends in Molecular Medicine·Rutger J HassinkLoren J Field
Aug 6, 2004·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Joseph C WuSanjiv S Gambhir
Aug 22, 2006·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Gwendolen Y ChangJoseph C Wu
May 26, 2005·Journal of Molecular and Cellular Cardiology·Mercy M DavidsonNithila D Isaac
Jun 1, 2001·The Annals of Thoracic Surgery·R M El OakleyM H Yacoub
Jun 25, 2002·The Annals of Thoracic Surgery·Jay G ShakeBradley J Martin
Dec 12, 2003·The Annals of Thoracic Surgery·Takeshiro FujiiRen-Ke Li
Dec 10, 1999·The Annals of Thoracic Surgery·M D Allen
Sep 12, 2003·Journal of Molecular and Cellular Cardiology·Mitsuhiro KudoMuhammad Ashraf
Oct 2, 2003·Journal of Molecular and Cellular Cardiology·W A LaFramboiseJ A Magovern
Oct 10, 2002·Brain Research Bulletin·George T SomogyiMichael B Chancellor
Mar 12, 2003·Journal of the American College of Cardiology·Rutger J HassinkPieter A Doevendans
Apr 8, 2003·Journal of the American College of Cardiology·Elina MinamiCharles E Murry
Apr 8, 2003·Journal of the American College of Cardiology·Philippe MenaschéDenis Duboc
May 15, 2002·Trends in Cardiovascular Medicine·Tetsuro SakaiJohnny Huard
Dec 28, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·B Z AtkinsD A Taylor
Jan 9, 2001·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·H Reinecke, C E Murry
Feb 27, 2004·Cardiovascular Radiation Medicine·Ron Waksman, Richard Baffour
Oct 4, 2002·Comptes rendus biologies·Philippe Menasché
Jul 10, 2009·Expert Reviews in Molecular Medicine·Steven J Joggerst, Antonis K Hatzopoulos
May 20, 2011·Molecular Pharmaceutics·Husnain Kh HaiderMuhammad Ashraf
Mar 22, 2002·Nature Reviews. Immunology·M Cascalho, J L Platt
Sep 28, 2004·Nature Biotechnology·Izhak KehatLior Gepstein
Feb 28, 2006·Nature Clinical Practice. Cardiovascular Medicine·Pedro L SánchezFrancisco Fernández-Avilés
Sep 28, 1999·Clinical and Experimental Pharmacology & Physiology·M Y AlexanderH M Prentice
Jun 9, 2001·The New England Journal of Medicine·A P BeltramiP Anversa
Jul 10, 2004·Human Gene Therapy·Hans ReineckeCharles E Murry
Sep 19, 2003·Tissue Engineering·P W ZandstraL J Field
Apr 14, 2009·Antioxidants & Redox Signaling·Husnain Kh HaiderMuhammad Ashraf
Feb 11, 2009·Antioxidants & Redox Signaling·Hyongbum KimYoung-Sup Yoon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.