PMID: 9435529Jan 22, 1998Paper

Skeletal unloading inhibits the in vitro proliferation and differentiation of rat osteoprogenitor cells

The American Journal of Physiology
P J KostenuikDaniel D Bikle

Abstract

Loss of weight bearing in the growing rat decreases bone formation, osteoblast numbers, and bone maturation in unloaded bones. These responses suggest an impairment of osteoblast proliferation and differentiation. To test this assumption, we assessed the effects of skeletal unloading using an in vitro model of osteoprogenitor cell differentiation. Rats were hindlimb elevated for 0 (control), 2, or 5 days, after which their tibial bone marrow stromal cells (BMSCs) were harvested and cultured. Five days of hindlimb elevation led to significant decreases in proliferation, alkaline phosphatase (AP) enzyme activity, and mineralization of BMSC cultures. Differentiation of BMSCs was analyzed by quantitative competitive polymerase chain reaction of cDNA after 10, 15, 20, and 28 days of culture. cDNA pools were analyzed for the expression of c-fos (an index of proliferation), AP (an index of early osteoblast differentiation), and osteocalcin (a marker of late differentiation). BMSCs from 5-day unloaded rats expressed 50% less c-fos, 61% more AP, and 35% less osteocalcin mRNA compared with controls. These data demonstrate that cultured osteoprogenitor cells retain a memory of their in vivo loading history and indicate that skeletal unloa...Continue Reading

References

Sep 22, 1978·Science·E R Morey, D J Baylink
Apr 1, 1990·The Journal of Cell Biology·P G StraussV Erfle
Dec 1, 1991·Archives of Biochemistry and Biophysics·E Kyeyune-NyombiD D Strong
Feb 1, 1987·Endocrinology·D D BikleE Morey-Holton
Nov 15, 1986·Analytical Biochemistry·R J GilliesM Denton
Feb 1, 1986·Endocrinology·R K GlobusE Morey-Holton
Jun 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K C Westerlind, R T Turner
Apr 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J J Van LoonJ P Veldhuijzen
Mar 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R ZhangD J Simmons
Mar 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S KeilaM Weinreb
Sep 27, 1993·Journal of Immunological Methods·S L ReinerR M Locksley
May 1, 1996·Endocrinology·Y Mikuni-TakagakiS Saito
Oct 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E I BarengoltsT G Unterman
Oct 1, 1979·Acta Astronautica·P C RambautG D Whedon

❮ Previous
Next ❯

Citations

Feb 14, 2002·Journal of Applied Physiology·Joseph S TashGeorge C Enders
May 30, 2008·Calcified Tissue International·Matthew J Silva, Michael D Brodt
Jul 14, 2020·Environmental Toxicology·Mi-Ae KangSee-Hyoung Park
Jun 6, 2000·American Journal of Physiology. Cell Physiology·J RubinX Fan
Oct 28, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Daniel S PerrienDana Gaddy
Oct 31, 2009·Journal of Applied Physiology·Hisataka KondoRuth K Globus
May 22, 2016·Physiological Genomics·Scott T CooperMarshall Hampton
May 25, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mohammad ShahnazariBernard P Halloran
Dec 11, 2020·Clinical and Experimental Pharmacology & Physiology·Young-Ju LimWon-Gu Jang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
E ZérathP J Marie
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
P J KostenuikDaniel D Bikle
© 2021 Meta ULC. All rights reserved