PMID: 3745943Feb 1, 1986Paper

Skin calcium-binding protein is a parvalbumin of the panniculus carnosus

The Journal of Investigative Dermatology
P Hawley-NelsonS H Yuspa

Abstract

Skin calcium-binding protein (SCaBP) is a calcium binding protein purified from whole rat skin. It has a molecular weight of approximately 12,000 daltons but migrates at Mr 13,000 on sodium dodecyl sulfate (SDS)-polyacrylamide gels. On nitrocellulose blots of SDS-polyacrylamide gels, 6 different antisera to SCaBP reacted equally well with SCaBP and parvalbumin (PV), an 11,500-dalton calcium-binding protein purified from rat skeletal muscle, which also migrates at Mr 13,000 on SDS-polyacrylamide gels. Rabbit antiserum to muscle PV also recognized both PV and SCaBP, and either protein absorbed specific antibodies against either antigen from both types of antisera. Soluble protein extracts from whole adult rat and mouse skin contained a Mr 13,000 protein which was recognized on nitrocellulose blots of SDS gels by both antisera. Blots of extracts from epidermis, dermis, whole skin, and skin scraped on the dermal side to remove hypodermal tissue revealed that the Mr 13,000 PV/SCaBP cross-reacting antigen was restricted to the hypodermal tissue removed by scraping. Immunofluorescent staining of Bouin-fixed skin sections with these antisera confirmed the localization of PV/SCaBP to the panniculus carnosus, a hypodermal muscle layer. N...Continue Reading

References

Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M W Berchtold, A R Means
Jul 1, 1985·The Biochemical Journal·J P MacManusM Yaguchi
Nov 28, 1980·Biochemical and Biophysical Research Communications·S H YuspaU Lichti
Mar 10, 1980·FEBS Letters·D LaouariS Balsan
Mar 1, 1981·The Journal of Investigative Dermatology·J H SauratS Balsan
Jan 1, 1980·Carcinogenesis·M F Kulesz-MartinS H Yuspa

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Citations

Apr 6, 1987·FEBS Letters·C P SchellingC W Heizmann
Jul 4, 2003·The Journal of Investigative Dermatology·Chun-Ming HuangDe-chu C Tang
Oct 1, 1986·The Journal of Investigative Dermatology·P Hawley-NelsonS H Yuspa

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