Skin derived precursor Schwann cell-generated acellular matrix modified chitosan/silk scaffolds for bridging rat sciatic nerve gap

Neuroscience Research
Changlai ZhuYun Gu

Abstract

Extracellular/acellular matrix has been attracted much research interests for its unique biological characteristics, and ACM modified neural scaffolds shows the remarkable role of promoting peripheral nerve regeneration. In this study, skin-derived precursors pre-differentiated into Schwann cells (SKP-SCs) were used as parent cells to generate acellular(ACM) for constructing a ACM-modified neural scaffold. SKP-SCs were co-cultured with chitosan nerve guidance conduits (NGC) and silk fibroin filamentous fillers, followed by decellularization to stimulate ACM deposition. This NGC-based, SKP-SC-derived ACM-modified neural scaffold was used for bridging a 10 mm long rat sciatic nerve gap. Histological and functional evaluation after grafting demonstrated that regenerative outcomes achieved by this engineered neural scaffold were better than those achieved by a plain chitosan-silk fibroin scaffold, and suggested the benefits of SKP-SC-derived ACM for peripheral nerve repair.

Citations

Jul 31, 2019·Biomaterials Science·A AlessandrinoG Freddi
Nov 19, 2019·Stem Cells Translational Medicine·Pihu MehrotraStelios T Andreadis
Nov 28, 2019·Journal of Biomaterials Science. Polymer Edition·Fangwen ZhaDemei Yu
Apr 26, 2019·Frontiers in Cellular Neuroscience·A BoeckerL Harhaus
Mar 3, 2020·Biomedical Materials·Mahboubeh JafarkhaniMohammad Ali Shokrgozar
Feb 27, 2021·Journal of Biomedical Materials Research. Part a·Jamileh SaremiSomayeh Ebrahimi-Barough
Jul 25, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ying MaXiaodong Cao

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