Skin imprinting in silica plates: a potential diagnostic methodology for leprosy using high-resolution mass spectrometry

Analytical Chemistry
Estela de Oliveira LimaRodrigo Ramos Catharino

Abstract

Leprosy is a chronic infectious disease caused by Mycobacterium leprae, which primarily infects macrophages and Schwann cells, affecting skin and peripheral nerves. Clinically, the most common form of identification is through the observation of anesthetic lesions on skin; however, up to 30% of infected patients may not present this clinical manifestation. Currently, the gold standard diagnostic test for leprosy is based on skin lesion biopsy, which is invasive and presents low sensibility for suspect cases. Therefore, the development of a fast, sensible and noninvasive method that identifies infected patients would be helpful for assertive diagnosis. The aim of this work was to identify lipid markers in leprosy patients directly from skin imprints, using a mass spectrometric analytical strategy. For skin imprint samples, a 1 cm(2) silica plate was gently pressed against the skin of patients or healthy volunteers. Imprinted silica lipids were extracted and submitted to direct-infusion electrospray ionization high-resolution mass spectrometry (ESI-HRMS). All samples were differentiated using a lipidomics-based data workup employing multivariate data analysis, which helped electing different lipid markers, for example, mycobacter...Continue Reading

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Citations

Mar 19, 2015·Analytical Chemistry·Estela de Oliveira LimaRodrigo Ramos Catharino
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Ewelina P Dutkiewicz, Pawel L Urban
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Feb 15, 2017·Analytical Chemistry·Ewelina P DutkiewiczPawel L Urban
Feb 24, 2018·Frontiers in Veterinary Science·Tatiane Melina GuerreiroRodrigo Ramos Catharino
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Apr 27, 2018·Frontiers in Bioengineering and Biotechnology·Carlos Fernando Odir Rodrigues MeloRodrigo Ramos Catharino
Jan 30, 2018·Frontiers in Pediatrics·Cibele Zanardi EstevesRodrigo Ramos Catharino
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