Skin irritant-induced cytotoxicity and prostaglandin E(2) release in human skin keratinocyte cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
J N LawrenceD J Benford

Abstract

Damage to the skin induced by chemical irritants is associated with the release of arachidonic acid metabolites, such as prostaglandin E(2) (PGE(2)) which plays an important role in epidermal inflammation. This study investigated cytotoxicity and release of PGE(2) in human epidermal keratinocytes following an 18 hr exposure of confluent cultures to various skin irritants. The concentration-dependent release of PGE, into the extracellular medium appeared to fall into two categories, which was reflective of possible mechanisms of action. Potent skin irritants, such as phorbol-12-myristate-13-acetate, benzalkonium chloride and tributyltin chloride, elicited an increase in extracellular PGE(2) levels at concentrations that did not produce overt cell damage (uptake of neutral red at these concentrations was comparable to control levels). Non-irritants (2-methoxyethanol and 2-butoxyethyl acetate) and two less severe irritants (sodium dodecyl sulfate and acetic acid) stimulated release of PGE(2) only at concentrations that compromised cellular integrity (uptake of neutral red was at least 50% lower than that of control cultures).

References

Jan 26, 1991·Lancet·J N BarkerB J Nickoloff
Mar 1, 1990·Experimental Cell Research·P J JensenJ Baird
Feb 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R Osborne, M A Perkins
Jan 1, 1996·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·P NeugebauerE Stennert
Apr 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J J van de Sandt, A A Rutten
Jun 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J N Lawrence, D J Benford
Jun 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J N LawrenceD J Benford
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F M DicksonD J Benford

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Citations

Jul 30, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jia-You FangChi-Feng Hung
Nov 2, 2004·Biological & Pharmaceutical Bulletin·Jia-You FangHsiao-Chin Cheng
Jun 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J N Lawrence
May 21, 2008·International Journal of Cosmetic Science·Andreas Natsch, Michael Wasescha
Jun 29, 2005·International Journal of Toxicology·UNKNOWN Cosmetic Ingredient Review Expert Panel
Oct 9, 2003·Reviews on Environmental Health·Denis Girard

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