Skin Keratins

Methods in Enzymology
Fengrong WangPierre A Coulombe

Abstract

Keratins comprise the type I and type II intermediate filament-forming proteins and occur primarily in epithelial cells. They are encoded by 54 evolutionarily conserved genes (28 type I, 26 type II) and regulated in a pairwise and tissue type-, differentiation-, and context-dependent manner. Keratins serve multiple homeostatic and stress-enhanced mechanical and nonmechanical functions in epithelia, including the maintenance of cellular integrity, regulation of cell growth and migration, and protection from apoptosis. These functions are tightly regulated by posttranslational modifications as well as keratin-associated proteins. Genetically determined alterations in keratin-coding sequences underlie highly penetrant and rare disorders whose pathophysiology reflects cell fragility and/or altered tissue homeostasis. Moreover, keratin mutation or misregulation represents risk factors or genetic modifiers for several acute and chronic diseases. This chapter focuses on keratins that are expressed in skin epithelia, and details a number of basic protocols and assays that have proven useful for analyses being carried out in skin.

Citations

Nov 28, 2016·American Journal of Human Genetics·Yinghong HeCristina Has
Sep 30, 2017·Proteomics. Clinical Applications·Henry A AdeolaNonhlanhla P Khumalo
Aug 29, 2017·PloS One·Pierre BalmerVidhya Jagannathan
Oct 18, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Galina WeingartenEfrat Wertheimer
Mar 28, 2020·Molecular Biology of the Cell·Kaylee SteenPierre A Coulombe
May 16, 2020·Frontiers in Cellular and Infection Microbiology·Meghan E Hermance, Saravanan Thangamani
Oct 4, 2020·Journal of Cell Science·Justin T JacobPierre A Coulombe
Dec 10, 2020·Journal of Developmental Biology·Duncan WotherspoonRyan F L O'Shaughnessy
Jan 5, 2021·Frontiers in Microbiology·Nonso E NnolimUchechukwu U Nwodo
Jan 19, 2021·Clinical and Experimental Immunology·P ChieosilapathamF Niyonsaba
Aug 19, 2021·Molecular Biology of the Cell·Pooja SharmaByung Min Chung

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