Skin-on-a-Chip: Transepithelial Electrical Resistance and Extracellular Acidification Measurements through an Automated Air-Liquid Interface

Genes
Frank A AlexanderJoachim Wiest

Abstract

Skin is a critical organ that plays a crucial role in defending the internal organs of the body. For this reason, extensive work has gone into creating artificial models of the epidermis for in vitro skin toxicity tests. These tissue models, called reconstructed human epidermis (RhE), are used by researchers in the pharmaceutical, cosmetic, and environmental arenas to evaluate skin toxicity upon exposure to xenobiotics. Here, we present a label-free solution that leverages the use of the intelligent mobile lab for in vitro diagnostics (IMOLA-IVD), a noninvasive, sensor-based platform, to monitor the transepithelial electrical resistance (TEER) of RhE models and adherent cells cultured on porous membrane inserts. Murine fibroblasts cultured on polycarbonate membranes were first used as a test model to optimize procedures using a custom BioChip encapsulation design, as well as dual fluidic configurations, for continuous and automated perfusion of membrane-bound cultures. Extracellular acidification rate (EAR) and TEER of membrane-bound L929 cells were monitored. The developed protocol was then used to monitor the TEER of MatTek EpiDermTMRhE models over a period of 48 hours. TEER and EAR measurements demonstrated that the designed...Continue Reading

References

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Citations

Feb 14, 2020·Biomedical Engineering Online·Qirui WuXiuqing Gong
Jun 9, 2020·Frontiers in Cellular and Infection Microbiology·Ka-Yee Grace ChoiRobert E W Hancock
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Christian SchmidtJoachim Wiest
Jun 24, 2020·Micromachines·Neda AzizipourAbdellah Ajji
Sep 4, 2020·International Journal of Pharmaceutics·R S N TavaresL R Gaspar
Jan 19, 2021·Small·Emily SutterbyElena Pirogova
Jul 15, 2021·APL Bioengineering·I RisueñoD Velasco
May 12, 2021·Annual Review of Analytical Chemistry·Patarajarin AkarapipadJeong-Yeol Yoon
Nov 27, 2018·Analytical Chemistry·Jong Hwan SungMichael L Shuler

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Methods Mentioned

BETA
biopsies
chip
chips

Software Mentioned

IVD
Data Acquisition and Link Application ( DALiA ) Client
IMOLA

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