Skin reactions to human papillomavirus (HPV) 16 specific antigens intradermally injected in healthy subjects and patients with cervical neoplasia

International Journal of Cancer. Journal International Du Cancer
Muriel van den HendeGemma G Kenter

Abstract

We have tested the safety and feasibility of a synthetic long peptide-based HPV16-specific skin test to detect cellular immune responses to HPV16 E2, E6 and E7 in vivo. Women with cervical neoplasia (n = 11) and healthy individuals (n = 19) were intradermally challenged with 8 different pools of HPV16 E2, E6 and E7 peptides. The skin test was safe as the injections were perceived as mildly painful and no adverse events were observed. The majority of skin reactions appeared significantly earlier in HPV16+ patients (<8 days) than in healthy subjects (8-25 days). The development of late skin reactions in healthy subjects was associated with the appearance of circulating HPV16-specific T cells and the infiltration of both HPV16-specific CD4+ Th1/Th2 and CD8+ T cells into the skin. These data show that the intradermal injection of pools of HPV16 synthetic long peptides is safe and results in the migration of HPV16-specific T cells into the skin as well as in an increase in the number of circulating HPV16-specific T cells. The use of this test to measure HPV16-specific immunity is currently tested in a low resource setting for the measurement of spontaneously induced T-cell responses as well as in our HPV16 vaccination trials for the...Continue Reading

References

Jan 1, 1975·International Archives of Allergy and Applied Immunology·J L Turk, D Parker
Dec 1, 1982·Cellular Immunology·L W PoulterG Panayi
Apr 1, 1995·The Journal of Infectious Diseases·M EvanderG Wadell
Jan 1, 1994·The Journal of General Virology·M A ChambersM A Stanley
Dec 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R E HuebnerJ B Bass
Mar 15, 1996·International Journal of Cancer. Journal International Du Cancer·D JenkinsS Gallivan
May 5, 1997·The American Journal of Medicine·L Koutsky
Nov 14, 1997·The New England Journal of Medicine·X W SunT C Wright
Feb 12, 1998·The New England Journal of Medicine·G Y HoR D Burk
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
Nov 11, 2003·American Journal of Infection Control·Ali AkcaySali Caglar
Nov 13, 2004·International Journal of Cancer. Journal International Du Cancer·Annemieke de JongCornelis J M Melief
Aug 19, 2005·International Journal of Cancer. Journal International Du Cancer·Mariëtte I E van PoelgeestSjoerd H van der Burg
Sep 16, 2006·International Journal of Cancer. Journal International Du Cancer·Anna-K Thomas-KaskelHendrik Veelken
Sep 19, 2006·Immunology Letters·Milica Vukmanovic-StejicArne N Akbar
Jul 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sjoerd H van der BurgRienk Offringa
Oct 24, 2007·International Journal of Cancer. Journal International Du Cancer·Sytse J PiersmaSjoerd H van der Burg

❮ Previous
Next ❯

Citations

Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marij J P WeltersSjoerd H van der Burg
Aug 13, 2013·Clinics and Research in Hepatology and Gastroenterology·Simon PernotJulien Taieb
Jun 12, 2012·Cancer Immunology, Immunotherapy : CII·Peggy J de Vos van SteenwijkSjoerd H van der Burg
Dec 21, 2010·Annual Review of Immunology·Ian H FrazerStephen R Mattarollo
Dec 5, 2012·Vaccine·Peter L SternMark H Einstein
Jan 14, 2011·Trends in Immunology·Sjoerd H van der BurgCornelis J M Melief
Mar 12, 2016·Nature Reviews. Cancer·Sjoerd H van der BurgCornelis J M Melief
Jul 22, 2009·International Journal of Cancer. Journal International Du Cancer·Ninke LeffersHans W Nijman
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Moniek HeusinkveldSjoerd H van der Burg
Sep 6, 2012·International Journal of Cancer. Journal International Du Cancer·Eliane C M ZeestratenCornelis J M Melief
Aug 1, 2009·International Journal of Cancer. Journal International Du Cancer·Yin Ling WooSjoerd H van der Burg
Sep 5, 2006·Virology·Suchitra JainMargaret A Stanley
Sep 19, 2008·Immunity·Cornelis J M Melief
Apr 8, 2017·Virus Research·Carlos SantosRui M Gil da Costa
Mar 8, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dinja OosterhoffTanja D de Gruijl
Jul 18, 2018·Frontiers in Immunology·Yugang GuoLi Tang
Nov 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Peggy J de Vos van SteenwijkSjoerd H van der Burg
Feb 4, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frank M SpeetjensSjoerd H van der Burg
Jul 3, 2021·Vaccines·Howard DonningerKavitha Yaddanapudi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.