Jun 5, 2003

Skin replacement with a collagen based dermal substitute, autologous keratinocytes and fibroblasts in burn trauma

Burns : Journal of the International Society for Burn Injuries
D Wisser, J Steffes


This case report describes the successful wound management of a 19-year-old patient with 76% of his body surface burnt. Due to the severity of the burns and the limited availability of native skin, 63% of the body surface were covered with Integra, a dermis equivalent. This material has been developed for the reconstruction of dermal structures and is designed as a bilayer. It consists of a modified, bovine collagen matrix and a silastic membrane facing toward the body surface. After vascularization of Integra, the epidermal skin replacement was created in a second step. In four different therapy procedures autologous, cultivated keratinocytes were combined with autologous fibroblast suspensions of various concentration and split-thickness skin as a 1:6 mesh graft. Rapid and largely complication-free wound healing could be achieved despite the limited availability of native skin. A correlation could be observed between the time of complete wound closure and the number of fibroblasts applied. The patient was monitored over a period of 1 year and showed good functional and cosmetic results.

  • References6
  • Citations38


Mentioned in this Paper

Bovine collagen
Specimen Type - Fibroblasts
Skin Substitutes
Pathologic Neovascularization
Wound Healing

About this Paper

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.