SKR-1, a homolog of Skp1 and a member of the SCF(SEL-10) complex, regulates sex-determination and LIN-12/Notch signaling in C. elegans.

Developmental Biology
Darrell J KillianDing Xue

Abstract

Sex-determination in Caenorhabditis elegans requires regulation of gene transcription and protein activity and stability. sel-10 encodes a WD40-repeat-containing F-box protein that likely mediates the ubiquitin-mediated degradation of important sex-determination factors. Loss of sel-10 results in a mild masculinization of hermaphrodites, whereas dominant alleles of sel-10, such as sel-10(n1074), cause a more severe masculinization, including a reversal of the life versus death decision in sex-specific neurons. To investigate about how sel-10 regulates sex-determination, we conducted a sel-10(n1074) suppressor screen and isolated a weak loss-of-function allele of skr-1, one of 21 Skp1-related genes in C. elegans. Skp1, Cullin, and F-box proteins, such as SEL-10, are components of the SCF E3 ubiquitin-ligase complex. We present genetic evidence that the sel-10(n1074) masculinization phenotype is dependent upon skr-1 and cul-1 activity. Furthermore, we show that the SKR-1(M140I) weak loss-of-function mutation interferes with SKR-1/SEL-10 binding. Unexpectedly, we found that the G567E substitution in SEL-10 caused by the n1074 allele impairs the binding of SEL-10 to SKR-1 and the dimerization of SEL-10, which may be important for S...Continue Reading

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Citations

May 11, 2011·Genome Biology·Antonio SarikasZhen-Qiang Pan
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Michael ChiorazziLouis M Staudt
Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·YueQin ZhangXueJun Hua
Mar 3, 2018·Proceedings of the National Academy of Sciences of the United States of America·Edyta KisielnickaChristian R Eckmann
Dec 12, 2018·Scientific Reports·Julian A PoushDavid S Fay

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