SLC25A22 promotes proliferation and metastasis by activating MAPK/ERK pathway in gallbladder cancer

Cancer Cell International
Pengcheng DuLu Fang

Abstract

SLC25A22, a member of mitochondrial carrier system (MCS) family encoding a mitochondrial glutamate transporter, has been reported to have vital roles in promoting proliferation and migration in cancer. Gallbladder cancer (GBC) is the most common biliary tract malignancy and has a poor prognosis. We aimed to determine the expression and function of SLC25A22 in GBC. Immunohistochemistry (IHC) staining analysis and quantitative real-time PCR (qRT-PCR) were conducted to determine the expression of SLC25A22 in GBC tissues. Human NOZ and GBC-SD cells were used to perform the experiments. The protein expression was detected by western-blot analysis. Cell viability was evaluated via CCK-8 assay and colony formation assay. Cell migration and invasion in vitro were investigated by wound healing and transwell assay. Annexin V/PI staining assay for apoptosis were measured by flow cytometry. The effect of SLC25A22 in vivo was conducted with subcutaneous xenograft. We indicated that the expression of SLC25A22 was significantly upregulated in GBC tumor tissues as well as cell lines. Downregulation of SLC25A22 inhibited GBC cell growth and proliferation in vitro and in vivo and also had an effect on metastasis of GBC cells through the EMT proc...Continue Reading

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Citations

Feb 6, 2020·Molecular Biology Reports·P BaichanE Nweke
Oct 9, 2020·Signal Transduction and Targeted Therapy·Xiaoling SongYingbin Liu
Oct 13, 2020·Journal of Zhejiang University. Science. B·Qian-Qian LiuFei-Yan Liu

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Methods Mentioned

BETA
xenograft
PCR
transfection
flow cytometry
xenografts

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