Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1

Proceedings of the National Academy of Sciences of the United States of America
Oliver WaliskoZ Ivics

Abstract

We used the Sleeping Beauty (SB) transposable element as a tool to probe transposon-host cell interactions in vertebrates. The Miz-1 transcription factor was identified as an interactor of the SB transposase in a yeast two-hybrid screen. Through its association with Miz-1, the SB transposase down-regulates cyclin D1 expression in human cells, as evidenced by differential gene expression analysis using microarray hybridization. Down-regulation of cyclin D1 results in a prolonged G(1) phase of the cell cycle and retarded growth of transposase-expressing cells. G(1) slowdown is associated with a decrease of cyclin D1/cdk4-specific phosphorylation of the retinoblastoma protein. Both cyclin D1 down-regulation and the G(1) slowdown induced by the transposase require Miz-1. A temporary G(1) arrest enhances transposition, suggesting that SB transposition is favored in the G(1) phase of the cell cycle, where the nonhomologous end-joining pathway of DNA repair is preferentially active. Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event.

References

Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·H MüllerM Strauss
May 1, 1993·Genes & Development·V BaldinG Draetta
Oct 1, 1995·Trends in Biochemical Sciences·S P Jackson, P A Jeggo
Sep 1, 1996·Current Biology : CB·M Emerman
May 9, 1997·The Journal of Biological Chemistry·T Zarkowska, S Mittnacht
Oct 6, 1997·The EMBO Journal·K PeukertM Eilers
Dec 1, 1996·Nature Biotechnology·D J LockhartE L Brown
Aug 4, 1999·Trends in Genetics : TIG·R H PlasterkZ Ivics
Aug 31, 2000·Journal of Molecular Biology·Z IzsvákR H Plasterk
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C Goodwin, D DiMaio
Apr 3, 2001·Nature Cell Biology·P StallerM Eilers
Nov 7, 2002·The Biochemical Journal·Louise Kime, Stephanie C Wright
Apr 25, 2003·Nucleic Acids Research·Hatem ZayedZoltán Ivics
Dec 3, 2003·Molecular and Cellular Biology·Kyoji HorieJunji Takeda
May 14, 2004·Journal of Virology·Chun-Jen Chen, Shinji Makino
Dec 8, 2004·Nature Cell Biology·Michael WanzelMartin Eilers

❮ Previous
Next ❯

Citations

Jul 8, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xin HuangXianzheng Zhou
Sep 17, 2010·Nature Reviews. Cancer·Neal G Copeland, Nancy A Jenkins
Jul 27, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Elise B PetersonWilliam J Bowers
Dec 12, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Oliver WaliskoZoltán Ivics
Jan 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kosuke YusaNancy L Craig
Apr 5, 2011·Human Molecular Genetics·Elena L AronovichPerry B Hackett
Mar 9, 2007·Nucleic Acids Research·Stephen R YantMark A Kay
Dec 11, 2013·Journal of Biomedical Science·Kristian Alsbjerg SkipperJacob Giehm Mikkelsen
Dec 6, 2007·Genome Biology·Yasunori SasakuraSatoko Awazu
Mar 15, 2014·PLoS Genetics·Yongming WangZsuzsanna Izsvák
Jul 13, 2006·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Stephen Fernando, Bradley S Fletcher
Jun 26, 2013·ELife·Corentin Claeys BouuaertRonald Chalmers
Dec 14, 2007·Annual Review of Genetics·Cédric Feschotte, Ellen J Pritham
Oct 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cédric Feschotte
Jun 12, 2012·Expert Opinion on Biological Therapy·Mario Di MatteoThierry Vandendriessche
Jun 24, 2015·Human Gene Therapy·Kristian Alsbjerg Skipper, Jacob Giehm Mikkelsen
Jul 25, 2014·Mobile Genetic Elements·Yujia Cai, Jacob Giehm Mikkelsen
Apr 9, 2011·BMC Biotechnology·Nicklas H StaunstrupJacob Giehm Mikkelsen
Jun 15, 2014·Biochemical and Biophysical Research Communications·Thirumala R TalluriWilfried A Kues
May 18, 2016·Chemical Reviews·Alison B Hickman, Fred Dyda
Oct 5, 2016·Critical Reviews in Biochemistry and Molecular Biology·Suneel A NarayanavariZsuzsanna Izsvák
Mar 8, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yasunori Sasakura
Sep 20, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Joanne R DohertyPaul E Mead
Jul 25, 2019·Frontiers in Oncology·Amy Guimaraes-YoungAdam J Dupuy
Jun 25, 2015·Microbiology Spectrum·Zoltán Ivics, Zsuzsanna Izsvák

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.