Slit diaphragm-reactive nephritogenic MAb 5-1-6 alters expression of ZO-1 in rat podocytes

The American Journal of Physiology
Hiroshi KawachiD J Salant

Abstract

Monoclonal antibody (MAb) 5-1-6 identifies a 51-kDa protein (p51) on rat podocyte foot processes and causes severe complement- and leukocyte-independent proteinuria when injected into rats. In the studies reported here, we used various immunohistological techniques to define the precise location of p51 and its relationship to ZO-1, a known component of the podocyte slit diaphragm in adult rat glomeruli. Our results demonstrate that p51 and ZO-1 lie close to each other on opposite sides of the podocyte plasma membrane at the point of insertion of the slit diaphragm: ZO-1 on the cytoplasmic face and p51 on the slit diaphragm and adjoining outer leaflet of the plasma membrane bordering the filtration slits. In addition to their geographic proximity, there appears to be a relationship between p51 and ZO-1. After MAb 5-1-6 injection, there was a progressive decline in stainable ZO-1 in the podocytes of heavily proteinuric rats. In addition, Western blot analysis of glomerular lysates showed that the decline in staining was due to a loss of immunoreactive ZO-1 rather than redistribution or diffusion of the protein. Simultaneously, the distribution of glomerular-bound MAb 5-1-6 became more clumped, apparently because of partial endocy...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H KuriharaM G Farquhar
May 1, 1992·The American Journal of Physiology·E WillottJ M Anderson
Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T OkasoraF Shimizu
Feb 1, 1992·Clinical and Experimental Immunology·H KawachiF Shimizu
Aug 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P MundelW Kriz
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B GumbinerD Apatira
Apr 1, 1988·Kidney International·D L Mendrick, H G Rennke
Jan 1, 1988·Methods in Enzymology·D J Salant, A V Cybulsky
May 19, 1988·Nature·S CitiJ Kendrick-Jones
Jul 1, 1985·The Journal of Experimental Medicine·T W Huang, J C Langlois
Feb 1, 1974·The Journal of Cell Biology·R Rodewald, M J Karnovsky
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·A SaitoM G Farquhar
Jun 1, 1993·Clinical and Experimental Immunology·M NarisawaF Shimizu
Nov 1, 1995·Anatomy and Embryology·P Mundel, W Kriz
Jan 1, 1961·The Journal of Experimental Medicine·M G FARQUHARG E PALADE

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Citations

Sep 24, 1999·Current Opinion in Nephrology and Hypertension·W Kriz, K V Lemley
Dec 31, 2002·Physiological Reviews·Hermann PavenstädtMatthias Kretzler
Mar 7, 2002·American Journal of Physiology. Renal Physiology·Huaiping YuanDavid J Salant
Sep 1, 2006·Journal of the American Society of Nephrology : JASN·Naoko MiyauchiHiroshi Kawachi
Mar 26, 2002·Journal of the American Society of Nephrology : JASN·Huaiping YuanDavid J Salant

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