Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance
Extending axons in the developing nervous system are guided in part by repulsive cues. Genetic analysis in Drosophila, reported in a companion to this paper, identifies the Slit protein as a candidate ligand for the repulsive guidance receptor Roundabout (Robo). Here we describe the characterization of three mammalian Slit homologs and show that the Drosophila Slit protein and at least one of the mammalian Slit proteins, Slit2, are proteolytically processed and show specific, high-affinity binding to Robo proteins. Furthermore, recombinant Slit2 can repel embryonic spinal motor axons in cell culture. These results support the hypothesis that Slit proteins have an evolutionarily conserved role in axon guidance as repulsive ligands for Robo receptors.
F-spondin: a gene expressed at high levels in the floor plate encodes a secreted protein that promotes neural cell adhesion and neurite extension
The axonal glycoprotein TAG-1 is an immunoglobulin superfamily member with neurite outgrowth-promoting activity.
Neural adhesion molecule L1 as a member of the immunoglobulin superfamily with binding domains similar to fibronectin
Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map
Mutations affecting growth cone guidance in Drosophila: genes necessary for guidance toward or away from the midline
commissureless controls growth cone guidance across the CNS midline in Drosophila and encodes a novel membrane protein
Hepatocyte growth factor/scatter factor is an axonal chemoattractant and a neurotrophic factor for spinal motor neurons
Distribution of the ten known laminin chains in the pathways and targets of developing sensory axons
Interference with axonin-1 and NrCAM interactions unmasks a floor-plate activity inhibitory for commissural axons
The family of the small leucine-rich proteoglycans: key regulators of matrix assembly and cellular growth
The chemorepulsive activity of secreted semaphorins is regulated by furin-dependent proteolytic processing
Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors
The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple aspects of axon guidance in C. elegans
Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline
Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening
Biochemical purification of a mammalian slit protein as a positive regulator of sensory axon elongation and branching
Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons
Vertebrate floor plate transiently expresses a compound recognized by antisera raised against subcommissural organ secretion
Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration
Alterations of ROBO1/DUTT1 and ROBO2 loci in early dysplastic lesions of head and neck: clinical and prognostic implications.
Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development
Development of precerebellar nuclei: instructive factors and intracellular mediators in neuronal migration, survival and axon pathfinding
Repulsive axon guidance: Abelson and Enabled play opposing roles downstream of the roundabout receptor
Axonal growth of the spinal cord interneurons expressing a homophilic adhesion molecule SC1 ectopically
Immunofluorescence laser confocal expression and localization study of rat nerve growth guidance cues Netrin-1 and Slit2 after spinal cord injury
Identification and characterization of Slitrk, a novel neuronal transmembrane protein family controlling neurite outgrowth
Rig-1 a new member of Robo family genes exhibits distinct pattern of expression during mouse development
Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.
Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.