SLO BK Potassium Channels Couple Gap Junctions to Inhibition of Calcium Signaling in Olfactory Neuron Diversification

PLoS Genetics
Amel AlqadahChiou-Fen Chuang

Abstract

The C. elegans AWC olfactory neuron pair communicates to specify asymmetric subtypes AWCOFF and AWCON in a stochastic manner. Intercellular communication between AWC and other neurons in a transient NSY-5 gap junction network antagonizes voltage-activated calcium channels, UNC-2 (CaV2) and EGL-19 (CaV1), in the AWCON cell, but how calcium signaling is downregulated by NSY-5 is only partly understood. Here, we show that voltage- and calcium-activated SLO BK potassium channels mediate gap junction signaling to inhibit calcium pathways for asymmetric AWC differentiation. Activation of vertebrate SLO-1 channels causes transient membrane hyperpolarization, which makes it an important negative feedback system for calcium entry through voltage-activated calcium channels. Consistent with the physiological roles of SLO-1, our genetic results suggest that slo-1 BK channels act downstream of NSY-5 gap junctions to inhibit calcium channel-mediated signaling in the specification of AWCON. We also show for the first time that slo-2 BK channels are important for AWC asymmetry and act redundantly with slo-1 to inhibit calcium signaling. In addition, nsy-5-dependent asymmetric expression of slo-1 and slo-2 in the AWCON neuron is necessary and s...Continue Reading

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Citations

Mar 31, 2017·Cellular and Molecular Life Sciences : CMLS·Yi-Wen HsiehChiou-Fen Chuang
Sep 25, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Amel AlqadahChiou-Fen Chuang
Aug 5, 2017·Journal of Cellular Physiology·Ekaterina Pchelintseva, Mustafa B A Djamgoz
Nov 9, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Amel AlqadahChiou-Fen Chuang
Aug 31, 2018·The Journal of Pharmacology and Experimental Therapeutics·Luisa L ScottJonathan T Pierce
Nov 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Amel AlqadahChiou-Fen Chuang
Oct 3, 2018·Communications Biology·Ichiro AokiIkue Mori

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

Fiji
ZEN
NIH ImageJ
Adobe Photoshop
AxioVision
Zeiss
ImageJ

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