Slow accumulation of acetylcholinesterase in rat brain during enzyme inhibition by repeated dosing with chlorpyrifos

Biochemical Pharmacology
S ChiappaStephen Brimijoin

Abstract

When given to rats, O,O'-diethyl-O-[3,5,6-trichloro-2-pyridyl]- phosphorothionate (chlorpyrifos), a common insecticide, causes an unusually lengthy dose-dependent fall in the activity of brain acetylcholinesterase (AChE; EC 3.1.1.7). To determine whether the slow recovery involves impaired AChE synthesis, experiments were designed to measure AChE activity, immunoreactive AChE protein (AChE-IR) and AChE mRNA. Male, Long-Evans rats, maintained at 350 +/- 5 g, were dosed (s.c.) weekly for 4 weeks with 0, 15, 30, or 60 mg/kg chlorpyrifos in peanut oil. Brain tissue was harvested 1, 3, 5, 7 and 9 weeks after treatment began. AChE activity was measured by Ellman assay, and AChE-IR was estimated by two-site ELISA using monoclonal antibodies to rat brain AChE. While AChE activity fell significantly at all times and doses, AChE-IR increased at 3 and 5 weeks in the two higher dosage groups. Larger increases of AChE-IR were observed after chlorpyrifos was administered for 4 weeks by the oral route. Northern blots quantified with reference to cyclophilin were consistent with stable levels of AChE mRNA. Overall, it appears that chronically reduced brain AChE activity after chlorpyrifos reflects sustained enzyme inhibition, not loss of enzym...Continue Reading

References

Nov 1, 1977·Biochemical Pharmacology·C M Cisson, B W Wilson
Dec 1, 1975·Journal of Neurochemistry·T L YakshH I Yamamura
Feb 1, 1992·RöFo : Fortschritte auf dem Gebiete der Röntgenstrahlen und der Nuklearmedizin·B FrennbyP Aspelin
Apr 8, 1991·Journal of Immunological Methods·G E GriesmannV A Lennon
Jun 15, 1990·Toxicology and Applied Pharmacology·J R Kemp, K B Wallace
Apr 1, 1989·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·G P CasaleJ J Connolly
May 1, 1988·DNA·P E DanielsonJ G Sutcliffe
Aug 1, 1987·Journal of Neurochemistry·S BrimijoinZ Rakonczay
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·P HammondS Brimijoin
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

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Citations

Sep 6, 2008·Brain : a Journal of Neurology·María-Salud García-AyllónJavier Sáez-Valero
May 24, 2014·Ecotoxicology and Environmental Safety·Renata Świergosz-KowalewskaAnna Halota
Mar 1, 2007·Journal of Neurochemistry·María-Salud García-AyllónJavier Sáez-Valero
Jun 17, 2008·Chemico-biological Interactions·María-Salud García-AyllónJavier Sáez-Valero
Jun 10, 2008·Chemico-biological Interactions·Wei SuXue-Jun Zhang
Feb 16, 2002·Regulatory Toxicology and Pharmacology : RTP·R C Cochran
Jul 20, 2011·Toxicology and Applied Pharmacology·Ellen G Duysen, Oksana Lockridge
Jul 12, 2001·Toxicology and Applied Pharmacology·D A JettG L McLemore
Mar 1, 1997·Human & Experimental Toxicology·M C LinternC B Ferry
Jan 1, 1997·Human & Experimental Toxicology·M C LinternC B Ferry
Jul 11, 2002·Cell Death and Differentiation·X J ZhangY F Shi
Aug 30, 2008·Critical Reviews in Toxicology·David L EatonPeter S Spencer
Jan 6, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Allison D FryerDavid A Jett

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