PMID: 11933046Apr 5, 2002Paper

Slow axonal transport of the cytosolic chaperonin CCT with Hsc73 and actin in motor neurons

Journal of Neuroscience Research
Gregory J BourkeM J Carden

Abstract

Molecular chaperones are well known for their role in facilitating the folding of nascent and newly synthesized proteins, but have other roles, including the assembly, translocation and renaturation of intracellular proteins. Axons are convenient tissues for the study of some of these other roles because they lack the capacity for significant protein synthesis. We examine the axonal transport of the cytosolic chaperonin containing T- complex polypeptide 1 (CCT) by labeling lumbar motor neurons with [35S]methionine and examining sciatic nerve proteins by 2-D gel electrophoresis and immunoblotting. All CCT subunits identifiable with specific antibodies, namely CCTalpha, CCTbeta, CCTgamma and CCTepsilon/CCTtheta; (the latter two subunits colocalized in analyses of rat nerve samples), appeared to be labeled in "slow component b" of axonal transport along with the molecular chaperone Hsc73 and actin, a major folding substrate for CCT. Our results are consistent with molecular chaperones having a post-translational role in maintaining the native form of actin during its slow transport to the axon terminal and ensuring its correct assembly into microfilaments.

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Citations

Jun 27, 2003·Cellular Immunology·Alfonso BarretoSusana Fiorentino
Oct 17, 2007·Immunology and Cell Biology·Di YuCarola G Vinuesa
May 16, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Subhojit RoyVirginia M-Y Lee
Aug 24, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Chang-Hwan BaeSeung-Tae Kim
Sep 27, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yong TangSubhojit Roy
Apr 21, 2007·The Journal of Comparative Neurology·Neeraja SammetaTimothy S McClintock
Jun 19, 2014·Biophysical Journal·Matan MusselUri Nevo
Aug 16, 2005·Journal of Neuroimmunology·Ricardo PalaciosPablo Villoslada
Jul 20, 2005·Molecular and Cellular Neurosciences·Ranjit S ShettyTimothy S McClintock
Jun 1, 2017·The Journal of Cell Biology·Archan GangulySubhojit Roy
Apr 11, 2019·Frontiers in Microbiology·Xue-Yang WangYang-Chun Wu
Aug 19, 2021·Neuroscience and Biobehavioral Reviews·Francesca LonghenaArianna Bellucci

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