Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle

Journal of Cell Science
Yusuke OnoShin'ichi Takeda

Abstract

Satellite cells are muscle stem cells that have important roles in postnatal muscle growth and adult muscle regeneration. Although fast- and slow-dividing populations in activated satellite cells have been observed, the functional differences between them remain unclear. Here we elucidated the relationship between proliferation behaviour and satellite cell function. To assess the frequency of cell division, satellite cells isolated from mouse EDL muscle were labelled with the fluorescent dye PKH26, stimulated to proliferate and then sorted by FACS. The vast majority of activated satellite cells were PKH26(low) fast-dividing cells, whereas PKH26(high) slow-dividing cells were observed as a minority population. The fast-dividing cells generated a higher number of differentiated and self-renewed cells compared with the slow-dividing cells. However, cells derived from the slow-dividing population formed secondary myogenic colonies when passaged, whereas those from the fast-dividing population rapidly underwent myogenic differentiation without producing self-renewing cells after a few rounds of cell division. Furthermore, slow-dividing cells transplanted into injured muscle extensively contributed to muscle regeneration in vivo. Id1...Continue Reading

References

Apr 10, 1996·Developmental Biology·E Schultz
Mar 1, 1996·Differentiation; Research in Biological Diversity·A BaroffioC R Bader
Dec 21, 2000·The Journal of Cell Biology·J R BeauchampP S Zammit
Jul 28, 2004·The Journal of Cell Biology·Peter S ZammitJonathan R Beauchamp
Sep 25, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Orna HalevyZipora Yablonka-Reuveni
Sep 6, 2005·Science·Didier MontarrasMargaret Buckingham
Jun 27, 2006·Nature Cell Biology·Vasily ShininShahragim Tajbakhsh
Nov 14, 2006·Developmental Biology·Raphaelle GrifonePascal Maire
Jun 2, 2007·Cell·Shihuan KuangMichael A Rudnicki
Sep 5, 2008·Journal of Cell Science·Peter S Zammit
Sep 23, 2008·Nature·Alessandra SaccoHelen M Blau
Nov 10, 2009·Cell Stem Cell·Hyung-song Nam, Robert Benezra
Jan 7, 2010·The Journal of Clinical Investigation·Francesco Saverio TedescoGiulio Cossu
Sep 21, 2010·Seminars in Cell & Developmental Biology·Stefano Biressi, Thomas A Rando
Nov 12, 2010·Science Translational Medicine·John K HallBradley B Olwin

❮ Previous
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Citations

Feb 24, 2015·Cell Reports·Yusuke OnoPeter S Zammit
Apr 5, 2016·Stem Cells International·Camila F AlmeidaMariz Vainzof
Nov 17, 2015·Nature Medicine·Nicolas A DumontMichael A Rudnicki
Mar 10, 2016·Nature Reviews. Molecular Cell Biology·Albert E Almada, Amy J Wagers
Mar 20, 2015·Tissue Engineering. Part B, Reviews·Melanie L HartJan E Brinchmann
Jun 9, 2016·Cellular and Molecular Life Sciences : CMLS·I Y ShadrinN Bursac
Jul 26, 2016·Frontiers in Cell and Developmental Biology·Sonia Alonso-MartinFrédéric Relaix
Dec 17, 2017·Journal of Cachexia, Sarcopenia and Muscle·Allan F PaganoGuillaume Py
Mar 14, 2018·The Journal of Clinical Investigation·Liwei XieHang Yin
Apr 27, 2018·Advanced Healthcare Materials·Alastair KhodabukusNenad Bursac
Jun 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ryo FujitaYusuke Ono
Oct 2, 2012·Nature·Joe V ChakkalakalAndrew S Brack
Nov 20, 2019·Wiley Interdisciplinary Reviews. Developmental Biology·Avnika A RupareliaPeter D Currie
Jan 1, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daiki SekoYusuke Ono
Jan 11, 2013·Physiological Reviews·Hang YinMichael A Rudnicki
Jan 30, 2020·Cytotechnology·Kirankumar B GudagudiKathryn H Myburgh
Sep 17, 2019·EMBO Reports·Kenichi SakamotoMinoru Takemoto
Aug 25, 2012·Current Opinion in Neurology·Francesco S Tedesco, Giulio Cossu
Apr 9, 2013·The FEBS Journal·Deborah Briggs, Jennifer E Morgan
Apr 15, 2015·Physiological Reports·Shinya MasudaYusuke Ono
Jun 6, 2013·The FEBS Journal·Didier MontarrasMargaret Buckingham
Mar 19, 2021·Frontiers in Cell and Developmental Biology·Yasuro FuruichiYasuko Manabe
Apr 4, 2021·The FEBS Journal·John F Bachman, Joe V Chakkalakal
Oct 11, 2014·Journal of Cell Science·Yu Xin WangMichael A Rudnicki
Apr 30, 2015·Development·Nicolas A DumontMichael A Rudnicki
Aug 11, 2021·Endocrinology and Metabolism·So-Ichiro Fukada, Ayasa Nakamura
Feb 27, 2021·Stem Cells·Morgan D ZumbaughDavid E Gerrard

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