Slow potentials encode intercellular coupling and insulin demand in pancreatic beta cells

Diabetologia
Fanny LebretonJochen Lang

Abstract

Ion fluxes constitute a major integrative signal in beta cells that leads to insulin secretion and regulation of gene expression. Understanding these electrical signals is important for deciphering the endogenous algorithms used by islets to attain homeostasis and for the design of new sensors for monitoring beta cell function. Mouse and human islets were cultured on multielectrode arrays (MEAs) for 3-13 days. Extracellular electrical activities received on each electrode were continuously amplified and recorded for offline characterisation. Differential band-pass filtering of MEA recordings of mouse islets showed two extracellular voltage waveforms: action potentials (lasting 40-60 ms) and very robust slow potentials (SPs, lasting 800-1,500 ms), the latter of which have not been described previously. The frequency of SPs directly correlated with glucose concentration, peaked at 10 mmol/l glucose and was further augmented by picomolar concentrations of glucagon-like peptide-1. SPs required the closure of ATP-dependent potassium channels as they were induced by glucose or glibenclamide but were not elicited by KCl-induced depolarisation. Pharmacological tools and the use of beta cell specific knockout mice showed that SPs reflec...Continue Reading

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Citations

Sep 2, 2016·Physiological Reviews·David G Nicholls
Dec 8, 2017·Physiological Reviews·Patrik Rorsman, Frances M Ashcroft
Jan 22, 2021·Diabetes·Aaron L GliebermanKevin Kit Parker
May 1, 2020·Biophysical Journal·Boris PodobnikMarko Jusup
Aug 22, 2020·Cell Chemical Biology·Julia HueyCarsten Schultz
Jul 20, 2021·American Journal of Physiology. Endocrinology and Metabolism·Andraž StožerJurij Dolenšek
May 17, 2019·The Journal of Physical Chemistry. a·Masakazu KuzeSatoshi Nakata

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