PMID: 9537585Apr 16, 1998Paper

Slow rate of free radical scavenging in the gastric antral mucosa of male Wistar rats: a possible mechanism of gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine

International Journal of Cancer. Journal International Du Cancer
T Mikuni, M Tatsuta

Abstract

We previously suggested that hydroxyl free radical (-OH) production may play a role in carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). MNNG-induced gastric cancer in rats and human gastric carcinoma occur most often in the antral mucosa and rarely in the normal fundic mucosa. We hypothesized that regional differences in anti-oxidant activity may be responsible. In the present study, we examined anti-oxidant activity by comparing the relative rates of reduction of a nitroxide free radical, 4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy (Tempol), in the antral and fundic mucosa of male Wistar rats using ESR. The relative rate of Tempol reduction was significantly slower in the antral portion of the wall than in the fundic portion when Tempol [4 x 10(-6) mole/mg wet weight of gastric wall] in HEPES buffer (pH 7.4) was spread over the mucosal surface of a section of the gastric wall. Addition of a sulfhydryl group modulator, N-ethylmaleimide, to the mucosal surface before treatment with Tempol removed the significant difference observed in the rates of reduction in the antral and fundic portions of the gastric wall. No signals were detected in the muscle layer. Our results indicate that the relative rate of f...Continue Reading

References

Mar 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·T MikuniM Kamachi
Mar 1, 1990·Archives of Biochemistry and Biophysics·K R MaplesR P Mason
Jan 1, 1985·Magnetic Resonance Imaging·W R CouetT N Tozer
Nov 18, 1969·Biochimica Et Biophysica Acta·M NagaoT Sugimura
May 1, 1973·The American Journal of Digestive Diseases·R G Strickland, I R Mackay
Aug 15, 1983·International Journal of Cancer. Journal International Du Cancer·M TatsutaH Taniguchi
Oct 9, 1981·Science·S SzaboP W Frankel

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