Slower cycling of nestin-positive cells in neurosphere culture

Neuroreport
Bao-Feng MaAndy Peng Xiang

Abstract

Neural stem cells are multipotent and self-renewing cells with important potential application in cell replacement therapy in brain damage. Many studies have shown that nestin-positive cells represent neural stem and progenitor cells in the central neural system. Here, we derived neural stem cells from the subventricular zone of a newborn nestin-promoter-driven green fluorescent protein mouse, and found that the percentage of nestin-positive cells decreased continuously at each passage in neurosphere culture. Using the relative proliferation ratio and relative division ratio analysis, we concluded that the slower cycling of nestin-positive cells was responsible for this decrease.

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Citations

Jun 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hyun-Jai ChoHyo-Soo Kim
Mar 2, 2010·The Journal of Biological Chemistry·Yi-Chao HsuIng-Ming Chiu
Oct 16, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yi-Chao HsuIng-Ming Chiu

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