slr1923 of Synechocystis sp. PCC6803 is essential for conversion of 3,8-divinyl(proto)chlorophyll(ide) to 3-monovinyl(proto)chlorophyll(ide).

Plant Physiology
M Rafiqul IslamHiroyuki Koike

Abstract

The deduced amino acid sequence of an slr1923 gene of Synechocystis sp. PCC6803 is homologous to archaean F(420)H(2) dehydrogenase, which acts as a soluble subcomplex of reduced nicotinamide adenine dinucleotide dehydrogenase complex I. In this study, the gene was inactivated and characteristics of the mutant were analyzed. The mutant grew slower than the wild type under 100 microE m(-2) s(-1) but did not grow under high light intensity (300 microE m(-2) s(-1)). The cellular content of chlorophyll was lower in the mutant, and the absorption spectrum showed a shift in the absorption peak of the Soret band to a longer wavelength by about 10 nm compared with the wild type. It was found, by high-performance liquid chromatography analysis, that the retention time of chlorophyll of the mutant is shorter than that of the wild type and that the peak wavelength of the Soret band was also shifted to a longer wavelength by 11 nm. Proton nuclear magnetic resonance analysis of the chlorophyll of the mutant revealed that the ethyl group of position 8 of ring B is replaced with a vinyl group. The spectrum indicates that the chlorophyll of the mutant is not a normal (3-vinyl)chlorophyll a but a 3,8-divinylchlorophyll a. These results strongly ...Continue Reading

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Citations

Jul 3, 2013·Photosynthesis Research·Md Rafiqul IslamHiroyuki Koike
Sep 5, 2015·Environmental Microbiology·Francisco RodríguezAnke Kremp
May 17, 2015·Biochimica Et Biophysica Acta·Pawel BrzezowskiBernhard Grimm
Feb 24, 2016·Journal of Bacteriology·Guangyu E ChenDaniel P Canniffe
Apr 4, 2020·The Journal of Biological Chemistry·Donald A BryantMartin J Warren
Aug 15, 2020·Frontiers in Microbiology·Ulysse GuyetLaurence Garczarek
Apr 14, 2016·The Journal of Biological Chemistry·Xiao Wang, Lin Liu
Aug 28, 2021·Biomolecules·Yi-Ting WangJei-Fu Shaw

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