Abstract
Salivary adenoid cystic carcinoma (SACC) is the most frequent salivary gland malignancy with a unique characteristic that has been named perineural invasion (PNI). EMMPRIN is a transmembrane glycoprotein that has been demonstrated to promote PNI in SACC. Slug, one of the most effective promoters of the epithelial-to-mesenchymal transition (EMT), has been found to be associated with PNI in SACC. The aim of the present study was to investigate the roles and relationships of Slug, EMMPRIN, and E-cadherin in the PNI process of SACC. The expression levels of Slug, EMMPRIN, and E-cadherin in 115 primary SACC cases were statistically analyzed by immunohistochemistry. Simultaneously, the SACC cell line SACC-83 was transfected with recombinant plasmids of silencing Slug (si-Slug) and/or silencing EMMPRIN (si-EMMPRIN). The functions of Slug and EMMPRIN in the EMT and PNI process were assessed by reverse transcription PCR (RT-PCR), western blotting, morphological observation, scratch test, migration assay, and in vitro perineural invasion assay. The immunohistochemical statistics revealed that the high expression of Slug and EMMPRIN and the low expression of E-cadherin were significantly associated with the PNI of SACC (P < 0.05). Slug ex...Continue Reading
References
Sep 2, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sandrine FaivrePatrice Berthaud
Sep 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mark AgulnikLillian L Siu
Sep 23, 2008·Oral Oncology·L D LocatiL Licitra
Dec 3, 2009·International Journal of Oral and Maxillofacial Surgery·Z TianJ Li
Mar 12, 2010·Oral Oncology·Yaling TangYu Chen
Aug 27, 2010·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Yaling TangYu Chen
Sep 21, 2010·Oral Oncology·Xinjie YangDelin Lei
Dec 15, 2010·The Lancet Oncology·Scott A LaurieDavid G Pfister
Jan 20, 2011·Cancer·Athanassios ArgirisArlene A Forastiere
May 3, 2011·Oncogene·J WuH Bian
Dec 2, 2011·The Journal of Biological Chemistry·Yong LiJian-Li Jiang
Dec 27, 2011·Cancer Biology & Therapy·Xinjie YangDelin Lei
Jan 21, 2014·Nature Reviews. Cancer·Frans van Roy
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Apr 9, 2014·Head & Neck·Moran AmitZiv Gil
May 27, 2014·Oral Oncology·Göran StenmanMattias K Andersson
Jul 22, 2014·Cell Biology International·Ning-Yu RuHuijie Bian
Aug 2, 2014·Advances in Cancer Research·G Daniel GrassBryan P Toole
Aug 30, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiao-Mei ZhouXia Han
Dec 3, 2014·Oral Oncology·Sen JiaDelin Lei
Dec 9, 2014·Cell Adhesion & Migration·Sarah Phillips, Charlotte Kuperwasser
Jan 28, 2015·Oncology Reports·Xiangming YangMoyi Sun
May 7, 2015·Oral Oncology·Andrés Coca-PelazAlfio Ferlito
May 13, 2015·Brazilian Journal of Otorhinolaryngology·Afonso Nóbrega DantasMaria de Lourdes Silva de Arruda Morais
May 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dan-tong Liu
Jul 15, 2015·Biochimica Et Biophysica Acta·Brijesh KumarSharmila A Bapat