SM22 a Plasma Biomarker for Human Transmural Intestinal Ischemia

Annals of Surgery
Dirk H S M SchellekensJoep P M Derikx

Abstract

To evaluate the diagnostic potential of smooth muscle protein of 22 kDa (SM22) as plasma biomarker for the detection of transmural intestinal ischemia. Acute mesenteric ischemia is an abdominal emergency requiring rapid diagnosis and treatment. Especially, detection of transmural damage is imperative because it mandates emergency surgery. Since early clinical and radiological signs are nonspecific, there is an urgent need for accurate biomarkers. SM22 is a potential marker for transmural damage because of its abundant expression in intestinal smooth muscles. SM22 concentrations were measured using a newly built enzyme-linked immunosorbent assay. SM22 release was assessed in plasma and intestinal tissue of rats subjected to intestinal ischemia. Blood and tissue were sampled at baseline and followed up to 24 hours of ischemia. Next, organ-specific SM22 arteriovenous concentration differences were studied in both rats and patients. Finally, plasma from patients with intestinal ischemia, other acute abdominal complaints, and healthy volunteers were tested for SM22. SM22 concentrations were significantly elevated in rats from 4 hours of ischemia onwards. Furthermore, SM22 plasma concentrations closely paralleled the histological inc...Continue Reading

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Citations

Jul 31, 2019·Scandinavian Journal of Gastroenterology·Xinyu WangWeiwei Ding
Aug 26, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Katell Peoc'hOlivier Corcos
Jan 5, 2021·Current Opinion in Critical Care·Annika Reintam BlaserYaseen M Arabi

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Methods Mentioned

BETA
imaging techniques
enzyme-linked immunosorbent assay
ELISA
blood drawn
protein assay
dissection
SMA

Software Mentioned

Graphpad Prism
GraphPad

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