Smad proteins physically interact with calmodulin.

The Journal of Biological Chemistry
C M ZimmermanL S Mathews

Abstract

The Smad family of intracellular proteins mediates signals generated by activin and other transforming growth factor beta-related proteins via specific heteromeric complexes of transmembrane receptor serine kinases (1, 2). xSmad2 has been implicated as an activin signal mediator that may participate in transcriptional regulation (3, 4). We have employed an interaction cloning strategy to identify xSmad2-binding proteins and found that calmodulin directly associated with Smads. xSmad2, generated either by in vitro translation or by overexpression in COS cells, specifically bound to calmodulin-agarose; the association was calcium-dependent and required xSmad2 N-terminal residues. In the same assay, xSmad1 and hSmads 2, 3, and 4 also bound to calmodulin-agarose. Furthermore, a calmodulin antagonist, W13, increased expression of the activin-inducible transcriptional reporter, 3TP-Lux, whereas overexpression of calmodulin suppressed this reporter. These observations demonstrate that Smad proteins interact with calmodulin in a calcium-dependent way through conserved N-terminal amino acids and suggest a role for calmodulin in regulating Smad function.

References

Oct 1, 1994·Cell Calcium·O BachsE Carafoli
Jan 1, 1995·Trends in Biochemical Sciences·P JamesE Carafoli
Apr 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A R Rhoads, F Friedberg
Dec 1, 1996·Trends in Genetics : TIG·J L Wrana, L Attisano

❮ Previous
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Citations

Jun 22, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J L Christian, T Nakayama
Nov 15, 2001·Pharmacology & Therapeutics·J Yue, K M Mulder
May 22, 2002·Cellular Signalling·Neus AgellPriam Villalonga
Sep 21, 2000·Trends in Endocrinology and Metabolism : TEM·S A Pangas, T K Woodruff
Jan 20, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·H YoshikawaK Takahashi
Dec 18, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keiji MiyazawaKohei Miyazono
Dec 6, 2000·European Journal of Biochemistry·S ItohP Ten Dijke
Aug 21, 2009·Annual Review of Genetics·Kevin J Parsons, R Craig Albertson
Jan 6, 2009·Biological & Pharmaceutical Bulletin·Naomi NishiiToshikatsu Nakabayashi
May 18, 2011·The Journal of Oral Implantology·Prasun ShahJames Rutkowski
Mar 4, 2008·Endocrine-related Cancer·Eric Glasgow, Lopa Mishra
Dec 1, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Tian-Fang LiDi Chen
Sep 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Immani Swapna, Laura N Borodinsky
Apr 6, 2011·Functional & Integrative Genomics·Joice de Faria PoloniDiego Bonatto
Mar 31, 2011·The International Journal of Biochemistry & Cell Biology·Einsley JanowskiMartin Morad
Sep 11, 2007·Experimental Cell Research·Jochen Haag, Thomas Aigner
Aug 28, 2007·Biochemical and Biophysical Research Communications·Scott L Cobbs, Jennifer L Gooch
Jul 12, 2005·Biochemical and Biophysical Research Communications·Agneta SimionescuNarendra Vyavahare
Mar 19, 2015·American Journal of Respiratory Cell and Molecular Biology·Luke J JanssenKjetil Ask
Jan 11, 2002·Developmental Biology·A von Bubnoff, K W Cho
Nov 23, 2000·Protein Expression and Purification·M Funaba, L S Mathews
Aug 16, 2016·American Journal of Respiratory and Critical Care Medicine·Haiyang TangJason X-J Yuan
Dec 26, 2006·Journal of Dental Research·L Häkkinen, A Csiszar
Jan 29, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Subhendu MukherjeeLuke J Janssen
Jul 6, 2004·The Journal of Comparative Neurology·Jane A FosterMiles Herkenham
Jan 16, 2010·Circulation Research·Jianyang DuLixia Yue
Jan 7, 1999·FEBS Letters·K HughesT Grundstrøm
Aug 24, 2002·The Journal of Biological Chemistry·Masayuki FunabaLawrence S Mathews
Sep 29, 2000·The Journal of Biological Chemistry·A Scherer, J M Graff
Jul 29, 2004·The Journal of Biological Chemistry·Juha SaarikettuThomas Grundström
Jan 1, 1998·Journal of Cellular Biochemistry·Michael E EngelHarold L Moses
Jan 30, 2002·Experimental Biology and Medicine·Ye-Guang ChenShao-Yao Ying
May 13, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shen ZhangJason X-J Yuan
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Tracy A McGowanKumar Sharma
Sep 27, 2002·Experimental Biology and Medicine·Corrine WeltAlan Schneyer
May 27, 2003·Journal of Cellular Physiology·Charles E Wenner, Shaochun Yan
May 15, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne R GockePeter A Calabresi
May 18, 1999·Biochemical and Biophysical Research Communications·R H XuH f Kung

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