Smad2 and Smad3 have differential sensitivity in relaying TGFβ signaling and inversely regulate early lineage specification

Scientific Reports
Ling LiuXiaoqing Zhang

Abstract

The transforming growth factor beta (TGFβ) related signaling is one of the most important signaling pathways regulating early developmental events. Smad2 and Smad3 are structurally similar and it is mostly considered that they are equally important in mediating TGFβ signals. Here, we show that Smad3 is an insensitive TGFβ transducer as compared with Smad2. Smad3 preferentially localizes within the nucleus and is thus sequestered from membrane signaling. The ability of Smad3 in oligomerization with Smad4 upon agonist stimulation is also impaired given its unique linker region. Smad2 mediated TGFβ signaling plays a crucial role in epiblast development and patterning of three germ layers. However, signaling unrelated nuclear localized Smad3 is dispensable for TGFβ signaling-mediated epiblast specification, but important for early neural development, an event blocked by TGFβ/Smad2 signaling. Both Smad2 and Smad3 bind to the conserved Smads binding element (SBE), but they show nonoverlapped target gene binding specificity and differential transcriptional activity. We conclude that Smad2 and Smad3 possess differential sensitivities in relaying TGFβ signaling and have distinct roles in regulating early developmental events.

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Citations

Feb 24, 2017·Journal of Cellular Biochemistry·Tahereh SoleimaniHossein Fallahi
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Datasets Mentioned

BETA
GSE76557

Methods Mentioned

BETA
nuclear translocation
nuclear
immunoprecipitation
Chip-Seq
transfection
PCR
electrophoresis
ChIP
Assay

Software Mentioned

macs14
bowtie
DAVID
SeqPos
Cistrome

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