SMAD3 and SP1/SP3 Transcription Factors Collaborate to Regulate Connective Tissue Growth Factor Gene Expression in Myoblasts in Response to Transforming Growth Factor β

Journal of Cellular Biochemistry
Gonzalo CórdovaEnrique Brandan

Abstract

Fibrotic disorders are characterized by an increase in extracellular matrix protein expression and deposition, Duchene Muscular Dystrophy being one of them. Among the factors that induce fibrosis are Transforming Growth Factor type β (TGF-β) and the matricellular protein Connective Tissue Growth Factor (CTGF/CCN2), the latter being a target of the TGF-β/SMAD signaling pathway and is the responsible for the profibrotic effects of TGF-β. Both CTGF and TGF are increased in tissues affected by fibrosis but little is known about the regulation of the expression of CTGF mediated by TGF-β in muscle cells. By using luciferase reporter assays, site directed mutagenesis and specific inhibitors in C2C12 cells; we described a novel SMAD Binding Element (SBE) located in the 5' UTR region of the CTGF gene important for the TGF-β-mediated expression of CTGF in myoblasts. In addition, our results suggest that additional transcription factor binding sites (TFBS) present in the 5' UTR of the CTGF gene are important for this expression and that SP1/SP3 factors are involved in TGF-β-mediated CTGF expression.

References

Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G BulfieldK J Moore
Feb 14, 1998·Cytokine & Growth Factor Reviews·G R Grotendorst
Jul 25, 1998·Genes & Development·Y G ChenJ Massagué
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Mar 4, 1999·Neuromuscular Disorders : NMD·P BernasconiR Mantegazza
Apr 25, 2000·The EMBO Journal·J Massagué, D Wotton
Jan 21, 2001·The Journal of Biological Chemistry·A HolmesA Leask
May 29, 2001·Molecular Pathology : MP·A LeaskD J Abraham
Mar 28, 2002·Physiological Reviews·Derek J BlakeKay E Davies
Jun 18, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Valerie ParadisPierre Bedossa
Jul 23, 2002·Nature Cell Biology·José G AbreuE M De Robertis
Sep 18, 2002·Kidney International·Youjun ChenAndrew Leask
Jul 31, 2003·The Journal of Biological Chemistry·Alan HolmesAndrew Leask
Jan 13, 2004·Physiological Reviews·Sophie B P Chargé, Michael A Rudnicki
Dec 3, 2005·Genes & Development·Joan MassaguéDavid Wotton
Nov 30, 2006·Journal of Cell Science·Andrew Leask, David J Abraham
Sep 12, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christine LangKarin Klingel
Nov 13, 2007·Journal of the Neurological Sciences·Guilian SunShigeru Tsuchiya
Dec 29, 2007·The Journal of Pathology·T A Wynn
Nov 17, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Ning LuDonald Gullberg
Oct 12, 2010·Arthritis and Rheumatism·Shangxi LiuAndrew Leask
Feb 3, 2011·Fibrogenesis & Tissue Repair·Qingjian WangDavid Yeowell
May 6, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sama F SleimanRajiv R Ratan
Jun 8, 2011·Journal of Cellular and Molecular Medicine·Claudio Cabello-VerrugioEnrique Brandan
Aug 10, 2011·The Journal of Pathology·María Gabriela MoralesEnrique Brandan
Feb 15, 2012·The Journal of Experimental Medicine·Makiko FujiiYoshitaka Sekido
Aug 18, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michel FaustherJonathan A Dranoff
Jan 25, 2013·International Journal of Molecular Sciences·Tian-Biao ZhouYuan-Han Qin
Jul 16, 2013·Expert Opinion on Therapeutic Targets·Andrew Leask
Aug 2, 2013·Human Molecular Genetics·Maria Gabriela MoralesEnrique Brandan
Sep 10, 2013·Molecular Neurobiology·Pedro CisternasNibaldo C Inestrosa

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Citations

Aug 5, 2015·Biochimica Et Biophysica Acta·Zhiwen FanYong Xu
Sep 28, 2016·Seminars in Cell & Developmental Biology·Antonio L Serrano, Pura Muñoz-Cánoves
Dec 31, 2016·Cell Biology International·Kamila DelaneyMalgorzata Zimowska
Sep 10, 2017·Journal of Cell Communication and Signaling·María Gabriela MoralesEnrique Brandan
Sep 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jennifer M PetrosinoFederica Accornero
Aug 28, 2016·Oncotarget·Natalia SmoktunowiczRachel C Chambers
Feb 24, 2020·Journal of Cell Communication and Signaling·Roger Valle-TenneyEnrique Brandan
Sep 16, 2015·Journal of Cardiovascular Pharmacology·Kamala SundararajDhandapani Kuppuswamy
Apr 21, 2020·Journal of the American Heart Association·Xiujie XieK Craig Kent
Jul 15, 2021·The Journal of Experimental Medicine·Andrea-Hermina GyörfiJörg H W Distler

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