Smads mediate signaling of the TGFbeta superfamily in normal keratinocytes but are lost during skin chemical carcinogenesis

Oncogene
W HeX J Wang

Abstract

The Smads are the signaling mediators of the TGFbeta superfamily. In the present study, we examined Smad expression in mouse epidermis and chemically-induced skin tumors. Mutations in Smad2 and -4 genes were also screened. Transcripts of Smad1 through -5 were constantly expressed in the epidermis regardless of changes in TGFbeta signaling, state of differentiation and stages of carcinogenesis. Smad7 transcripts were barely detectable in keratinocytes, but were induced by TGFbeta1 treatment and in chemically-induced skin tumors. At the protein level, Smad1 was detected throughout the epidermis, whereas Smad2 through -5 exhibited greater levels in suprabasal layers than basal keratinocytes. In cultured keratinocytes, Smad2, -3 and -4 underwent nuclear translocation upon TGFbeta1 treatment. Furthermore, nuclear translocation of Smads correlated with decreased BrdU labeling in proliferative keratinocytes. Although no mutations were detected in the Smad2 and -4 genes in tumors, proteins of Smad1 through -5 were partially or completely lost in carcinomas. These data document that Smads are expressed at high levels in the epidermis and mediate signaling of the TGFbeta superfamily. During skin carcinogenesis, loss of Smad1 through -5 a...Continue Reading

References

Jan 1, 1992·Pharmacology & Therapeutics·J DiGiovanni
Jan 1, 1991·Acta neurochirurgica·I BaileyJ Ohman
Jan 1, 1988·Annals of the New York Academy of Sciences·D D Bikle, S Pillai
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C M AldazT J Slaga
Jan 1, 1987·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·A B Roberts, M B Sporn
Jan 1, 1995·Teratogenesis, Carcinogenesis, and Mutagenesis·M BlessingB L Hogan
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K SellheyerD R Roop
Aug 1, 1996·Journal of Cellular Physiology·Z RouguiM Rizk-Rabin
Jan 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A NadeauR Blouin
Apr 1, 1997·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·C A MoskalukS E Kern
Jan 1, 1997·Gut·A T HoqueS E Kern
Oct 23, 1997·Nature·T ImamuraK Miyazono
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·X LiuH F Lodish
Feb 14, 1998·The Journal of Biological Chemistry·C M ZimmermanL S Mathews
Mar 28, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·X T KongY Hayashi
Jan 9, 1999·Journal of Biochemistry·M Kawabata, K Miyazono
Jan 19, 1999·Genes, Chromosomes & Cancer·T JonsonM Höglund
Apr 10, 1999·Genes & Development·M KretzschmarJ Massagué

❮ Previous
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Citations

Jun 7, 2012·Clinical Oral Investigations·Yuk-Kwan ChenLi-Min Lin
Feb 16, 2010·Journal of Molecular Neuroscience : MN·Yu Wang, Aviva J Symes
Mar 17, 2004·Experimental Cell Research·Louise SmithNicholas A Saunders
Jan 16, 2002·Current Opinion in Genetics & Development·Lalage M Wakefield, Anita B Roberts
Mar 26, 2003·Cytokine & Growth Factor Reviews·Allen G LiXiao Jing Wang
Feb 25, 2006·Nature Reviews. Cancer·Sam M Janes, Fiona M Watt
Dec 30, 2011·Cell & Bioscience·Lu ZhuYan Chen
Dec 10, 2013·International Journal of Molecular Sciences·Carmine StolfiGiovanni Monteleone
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·X LiuA Glick
Jan 21, 2010·International Journal of Biological Sciences·Guan Yang, Xiao Yang
Apr 4, 2009·Experimental and Molecular Pathology·Aimin LengGuiying Zhang
Sep 1, 2007·The Journal of Investigative Dermatology·John M LamarC Michael DiPersio
Dec 17, 2003·The Journal of Investigative Dermatology·Andreas KaratsaidisKarl Schenck
Jan 22, 2003·The Journal of Investigative Dermatology·Vladimir A Botchkarev
Mar 14, 2008·The Journal of Investigative Dermatology·Philip OwensXiao-Jing Wang
Jul 4, 2003·The Journal of Investigative Dermatology·Shinsuke TakagawaJohn Varga
May 15, 2007·European Journal of Cell Biology·Borut KlopcicManfred Blessing
May 5, 2004·Genesis : the Journal of Genetics and Development·Kin Ming KwanRichard R Behringer
May 5, 2007·Molecular Carcinogenesis·Sophia BornsteinXiao-Jing Wang
Sep 27, 2008·The EMBO Journal·Pascal DescarguesMichael Karin
Jan 21, 2006·Birth Defects Research. Part C, Embryo Today : Reviews·Judith A MackEdward V Maytin
Sep 29, 2011·The American Journal of Pathology·Gangwen HanXiao-Jing Wang
Apr 7, 2005·Mechanisms of Ageing and Development·Kwang-Ho HanKyu-Han Kim
Aug 15, 2006·Biochimica Et Biophysica Acta·Katerina Pardali, Aristidis Moustakas
Jun 1, 2005·Experimental Cell Research·Sunil K HalderPran K Datta
Dec 25, 2004·Differentiation; Research in Biological Diversity·Vladimir A Botchkarev, Andreij A Sharov
Aug 25, 2009·The American Journal of Pathology·Andrey A SharovVladimir A Botchkarev
May 7, 2013·Mutation Research·Minli WangFrancis A Cucinotta
Sep 5, 2006·Developmental Cell·Gangwen HanXiao-Jing Wang
Jul 19, 2014·Clinical Oral Investigations·Sopee PoomsawatPaisarn Vejchapipat
Jan 9, 2015·Protein & Cell·Li BianXiao-Jing Wang
Feb 15, 2001·Microscopy Research and Technique·X J Wang
May 11, 2004·Veterinary Pathology·S H Tannehill-GreggM Weinstein
Nov 14, 2017·American Journal of Nephrology·Sagar U NigwekarDonald B Bloch
Nov 28, 2002·International Journal of Impotence Research·N F Gonzalez-CadavidJ Rajfer
Feb 11, 2003·The Journal of Biological Chemistry·Liyu WuXu Cao
May 15, 2020·Journal of Cellular and Molecular Medicine·Hanyan DingYan Yang

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