SmaI macrorestriction analysis of Italian isolates of erythromycin-resistant Streptococcus pyogenes and correlations with macrolide-resistance phenotypes

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Sandro RipaPietro E Varaldo

Abstract

High rates of erythromycin resistance among Streptococcus pyogenes strains have been reported in Italy in the last few years. In this study, 370 erythromycin-resistant (MIC, > or = 1 microg/mL) Italian isolates of this species obtained in 1997-1998 from throat swabs from symptomatic patients were typed by analyzing SmaI macrorestriction fragment patterns by pulsed-field gel electrophoresis (PFGE). Among the typable isolates (n = 341; the genomic DNA of the remaining 29 isolates was not restricted by SmaI), 48 distinct PFGE types were recognized, of which 31 were recorded in only one isolate (one-strain types). Fifty-two percent of typable isolates fell into three type clusters and 75% into six, suggesting that erythromycin-resistant group A streptococci circulating in Italy are polyclonal, but the majority of them probably derives from the spread of a limited number of clones. In parallel experiments, the 370 test strains were characterized for the macrolide resistance phenotype: 80 were assigned to phenotype cMLS, 89 to phenotype iMLS-A, 33 to phenotype iMLS-B, 11 to phenotype iMLS-C, and 157 to phenotype M. There was a close correlation between these phenotypic data and the genotypic results of PFGE analysis, the vast majorit...Continue Reading

References

Jan 30, 1992·The New England Journal of Medicine·H SeppäläT Klaukka
Dec 1, 1994·The Pediatric Infectious Disease Journal·K FujitaT Murai
Dec 1, 1993·The Journal of Antimicrobial Chemotherapy·H SeppäläP Huovinen
Oct 1, 1995·Antimicrobial Agents and Chemotherapy·P R HsuehJ J Wu
Jan 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C DavidD Ingrand
Jan 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E L Kaplan
Oct 24, 1997·The Journal of Antimicrobial Chemotherapy·M BorzaniF Varotto
Apr 30, 1999·Emerging Infectious Diseases·E Perez-TralleroM de Pablos
Jun 29, 1999·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S ValisenaR Fontana
Nov 24, 1999·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J Melo-Cristino, M L Fernandes
Dec 10, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P E VaraldoG Tempera

❮ Previous
Next ❯

Citations

May 11, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Claudia ZampaloniSandro Ripa
Apr 23, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·R E C d'OliveiraA C D Castro
Sep 24, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Maria Pia MontanariPietro Emanuele Varaldo
Nov 13, 2008·Antimicrobial Agents and Chemotherapy·Pietro E VaraldoEleonora Giovanetti
Jan 26, 2011·Antimicrobial Agents and Chemotherapy·Marina MingoiaMaria Pia Montanari
Feb 21, 2008·Antimicrobial Agents and Chemotherapy·Ileana CochettiMaria Pia Montanari
Jan 31, 2007·Antimicrobial Agents and Chemotherapy·Andrea BrencianiEleonora Giovanetti
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Eleonora GiovanettiPietro Emanuele Varaldo
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Giordano DicuonzoBernard Beall
Jun 25, 2003·Antimicrobial Agents and Chemotherapy·Maria P MontanariPietro E Varaldo
Mar 13, 2009·Journal of Clinical Microbiology·Luca Agostino VitaliSandro Ripa
Feb 5, 2002·Journal of Clinical Microbiology·Luca A VitaliSandro Ripa
Aug 20, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S IoannidouA C Vatopoulos
Mar 18, 2005·The Journal of Antimicrobial Chemotherapy·Eleonora GiovanettiPietro E Varaldo
Mar 7, 2003·Journal of Medical Microbiology·Maria Celeste Nunes de MeloBernadete Teixeira Ferreira-Carvalho
Feb 21, 2021·Applied and Environmental Microbiology·Simona FioritiEleonora Giovanetti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.