Small-angle X-ray scattering characteristics of mouse brain: Planar imaging measurements and tomographic imaging simulations

PloS One
Mina ChoiAldo Badano

Abstract

Small-angle x-ray scattering (SAXS) imaging can differentiate tissue types based on their nanoscale molecular structure. However, characterization of the coherent scattering cross-section profile of relevant tissues is needed to optimally design SAXS imaging techniques for a variety of biomedical applications. Reported measured nervous tissue x-ray scattering cross sections under a synchrotron source have had limited agreement. We report a set of x-ray cross-section measurements obtained from planar SAXS imaging of 1 mm thick mouse brain (APP/PS1 wild-type) coronal slices using an 8 keV laboratory x-ray source. Two characteristic peaks were found at 0.96 and 1.60 nm-1 attributed to myelin. The peak intensities varied by location in the slice. We found that regions of gray matter, white matter, and corpus callosum could be segmented by their increasing intensities of myelin peaks respectively. Measured small-angle x-ray scattering cross sections were then used to define brain tissue scattering properties in a GPU-accelerated Monte Carlo simulation of SAXS computed tomography (CT) using a higher monochromatic x-ray energy (20 keV) to study design trade-offs for noninvasive in vivo SAXS imaging on a small-animal head including rad...Continue Reading

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Citations

May 27, 2021·Biomedical Physics & Engineering Express·Mina ChoiAldo Badano

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Methods Mentioned

BETA
x-ray scattering
imaging techniques
biopsy

Software Mentioned

SAXStreat
MC
PENELOPE
GPU

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