PMID: 11334359May 4, 2001Paper

Small blood volumes from children for quantitative sotalol determination using high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
Stephanie LäerH Scholz

Abstract

A sensitive high-performance liquid chromatographic method using fluorescence detection has been developed for sotalol determination in small plasma samples of children and newborns with limited blood volume. In sample sizes of 100 microl of plasma, sotalol was extracted using an internal standard and solid-phase extraction columns. Chromatographic separation was performed on a Spherisorb C6 column of 150x4.6 mm I.D. and 5 microm particle size at ambient temperature. The mobile phase consisted of acetonitrile-15 mM potassium phosphate buffer (pH 3.0) (70:30, v/v). The excitation wavelength was set at 235 nm, emission at 300 nm. The flow-rate was 1 ml/min. Sotalol and the internal standard atenolol showed recoveries of 107+/-8.9 and 97+/-8.1%, respectively. The linearity range for sotalol was between 0.07 and 5.75 microg/ml, the limit of quantitation 0.09 microg/ml. Precision values expressed as percent relative standard deviation of intra-assay varied between 0.6 and 13.6%, that of inter-assay between 2.4 and 14.4%. Accuracy varied between 86.1 and 109.8% (intra-assay) and 95.4 and 103.3% (inter-assay). Other clinically used antiarrhythmic drugs did not interfere. As an application of the assay, sotalol plasma concentrations in...Continue Reading

References

Sep 1, 1989·Journal of Chromatography·J M PoirierG Cheymol
Jun 7, 1996·Journal of Chromatography. B, Biomedical Applications·S LäerN Zimmermann
Jul 8, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M Zilli, L Zorzenon

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Citations

Jun 29, 2005·European Journal of Clinical Pharmacology·J J LiljaP J Neuvonen
Aug 30, 2005·Journal of Pharmacological and Toxicological Methods·Jan-Peer Elshoff, Stephanie Läer
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Veli-Pekka RantaArto Urtti
Jul 13, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michael SchlauchAugust W Frahm
Oct 16, 2004·Biomedical Chromatography : BMC·Stefanie AlbersStephanie Läer
May 17, 2006·Basic & Clinical Pharmacology & Toxicology·Jari J LiljaPertti J Neuvonen
Jan 15, 2015·Biomedical Chromatography : BMC·Zhiping FengXianhua Zhang
Nov 2, 2006·The Journal of Toxicological Sciences·Mitsuyasu TaboShigeo Ito
Dec 21, 2005·Basic & Clinical Pharmacology & Toxicology·Jari J LiljaPertti J Neuvonen
Nov 13, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Irena BaranowskaJacek Baranowski

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