Small-conductance Ca(2+)-dependent K+ channels activated by ATP in murine colonic smooth muscle

The American Journal of Physiology
S D KohK M Sanders

Abstract

The patch-clamp technique was used to determine the ionic conductances activated by ATP in murine colonic smooth muscle cells. Extracellular ATP, UTP, and 2-methylthioadenosine 5'-triphosphate (2-MeS-ATP) increased outward currents in cells with amphotericin B-perforated patches. ATP (0.5-1 mM) did not affect whole cell currents of cells dialyzed with solutions containing ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. Apamin (3 x 10(-7) M) reduced the outward current activated by ATP by 32 +/- 5%. Single channel recordings from cell-attached patches showed that ATP, UTP, and 2-MeS-ATP increased the open probability of small-conductance, Ca(2+)-dependent K+ channels with a slope conductance of 5.3 +/- 0.02 pS. Caffeine (500 microM) enhanced the open probability of the small-conductance K+ channels, and ATP had no effect after caffeine. Pyridoxal phosphate 6-azophenyl-2',4'-disulfonic acid tetrasodium (PPADS, 10(-4) M), a nonselective P2 receptor antagonist, prevented the increase in open probability caused by ATP and 2-MeS-ATP. PPADS had no effect on the response to caffeine. ATP-induced hyperpolarization in the murine colon may be mediated by P2y-induced release of Ca2+ from intracellular stores and acti...Continue Reading

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Citations

Nov 30, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·A N HolmG Farrugia
Feb 24, 2001·Journal of Autonomic Pharmacology·F Vogalis, T K Smith
Sep 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·S RoB Horowitz
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Feb 15, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Laszlo KölesPeter Illes
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Aug 14, 2009·Fundamental & Clinical Pharmacology·Nilce Mitiko Matsuda, Steven M Miller
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