Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innate lymphoid cell-dominant allergic airway inflammation through delivery of miR-146a-5p

Journal of Extracellular Vesicles
Shu-Bin FangQing-Ling Fu

Abstract

Group 2 innate lymphoid cells (ILC2s) are recently reported to play a more critical role in allergic diseases. We previously identified that mesenchymal stromal cells (MSCs) elicited therapeutic effects on allergic airway inflammation. Small extracellular vesicles (sEV) derived from MSCs possess striking advantages including low immunogenicity and high biosafety, and is extremely promising cell-free therapeutic agents. However, the effects of MSC-sEV on ILC2s are still unclear. Additionally, scalable isolation protocols are required for the mass production of homogenous MSC-sEV especially in clinical application. We previously reported that induced pluripotent stem cells-derived MSCs were the ideal cellular source for the large preparation of MSC-sEV. Here we developed a standardized scalable protocol of anion-exchange chromatography for isolation of MSC-sEV, and investigated the effects of MSC-sEV on ILC2 function from patients with allergic rhinitis and in a mouse ILC2-dominant asthma model. The characterization of MSC-sEV was successfully demonstrated in terms of size, morphology and specific markers. Using flow cytometry and human Cytokine Antibody Array, MSC-sEV but not fibroblasts-sEV (Fb-sEV) were found to significantly ...Continue Reading

References

Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Marina ColomboClotilde Théry
Nov 5, 2014·Nature Immunology·Laura J SimpsonK Mark Ansel
Mar 1, 2015·Genomics, Proteomics & Bioinformatics·Jian ZhangShuangli Mi
Mar 15, 2015·Seminars in Cell & Developmental Biology·Ruenn Chai LaiSai Kiang Lim
Aug 9, 2015·Current Opinion in Immunology·Heather H Pua, K Mark Ansel
Dec 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Dong-Ki KimDarwin J Prockop
Aug 6, 2016·The Journal of Allergy and Clinical Immunology·Kristina JohanssonMadeleine Rådinger
Sep 2, 2016·Stem Cell Research & Therapy·Jeffrey L SpeesCarl A Gregory
Jul 12, 2017·The Journal of Experimental Medicine·Alexander V MisharinHarris Perlman
Sep 14, 2017·Nature·Antonia WallrappVijay K Kuchroo
Sep 15, 2017·American Journal of Respiratory and Critical Care Medicine·Tara F CarrMonica Kraft
Sep 18, 2017·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Hua ZhongQing-Ling Fu
Nov 11, 2017·The Journal of Experimental Medicine·Priti B SinghK Mark Ansel
Oct 26, 2018·Frontiers in Immunology·Martin D KnolleAndrew N J McKenzie

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Citations

Jul 10, 2020·Respiratory Research·Aradhana MohanNavneet K Dhillon
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Zhiyi HuangSong Guo Zheng
Jun 27, 2020·Stem Cells International·Liwei SunDongdong Zhu
Sep 20, 2020·International Journal of Molecular Sciences·Jung Hwa Ko, Joo Youn Oh
Jan 21, 2021·International Journal of Nanomedicine·Kai ChenXin Wang
Jan 23, 2021·International Journal of Molecular Sciences·José A CañasVictoria del Pozo
Feb 6, 2021·Diagnostics·Katarzyna PiszczatowskaMirosław J Szczepański
Dec 15, 2020·World Journal of Stem Cells·Hui-Kang XuCharlie Xiang
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Marietta HerrmannSusanne Grässel
Jan 13, 2021·International Journal of Molecular Sciences·Laura PastorDavid Sanz-Rubio
Sep 12, 2020·Molecular Therapy. Methods & Clinical Development·Laetitia S GasparLuís Pereira de Almeida
Mar 3, 2021·Stem Cell Reviews and Reports·Marlena TyneckaAndrzej Eljaszewicz
Mar 7, 2021·Journal of Clinical Medicine·Byung-Chul LeeKyung-Rok Yu
Mar 5, 2021·Frontiers in Cell and Developmental Biology·Soraia C AbreuPatricia R M Rocco
Mar 13, 2021·Journal of Extracellular Vesicles·Vivian V T NguyenBas W M van Balkom
Mar 16, 2021·Frontiers in Pharmacology·Wumei WeiXiaohong Tian
Feb 4, 2021·Annual Review of Immunology·Noe Rodriguez-RodriguezAndrew N J McKenzie
May 8, 2021·Frontiers in Pharmacology·Andressa Daronco CeretaRenata Kelly da Palma
May 11, 2021·Nanoscale·Jianghong HuangYujie Liang
Oct 24, 2020·Molecular Immunology·Cong WangQing-Ling Fu
Aug 23, 2021·Advanced Drug Delivery Reviews·Simon StaubachBernd Giebel

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Methods Mentioned

BETA
protein
enzyme-linked immunosorbent assay
protein assay
Transfection
PCR
flow cytometry
Transmission electron microscopy
density
ELISA
Assay

Software Mentioned

GraphPad
Prism
miRTarBase
DAVID Resources
miRWalkGO
TargetScan
Nanosight

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