Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential

Basic Research in Cardiology
Kaloyan TakovSean M Davidson

Abstract

Mesenchymal stromal cells (MSCs) exhibit antiapoptotic and proangiogenic functions in models of myocardial infarction which may be mediated by secreted small extracellular vesicles (sEVs). However, MSCs have frequently been harvested from aged or diseased patients, while the isolated sEVs often contain high levels of impurities. Here, we studied the cardioprotective and proangiogenic activities of size-exclusion chromatography-purified sEVs secreted from human foetal amniotic fluid stem cells (SS-hAFSCs), possessing superior functional potential to that of adult MSCs. We demonstrated for the first time that highly pure (up to 1.7 × 1010 particles/µg protein) and thoroughly characterised SS-hAFSC sEVs protect rat hearts from ischaemia-reperfusion injury in vivo when administered intravenously prior to reperfusion (38 ± 9% infarct size reduction, p < 0.05). SS-hAFSC sEVs did not protect isolated primary cardiomyocytes in models of simulated ischaemia-reperfusion injury in vitro, indicative of indirect cardioprotective effects. SS-hAFSC sEVs were not proangiogenic in vitro, although they markedly stimulated endothelial cell migration. Additionally, sEVs were entirely responsible for the promigratory effects of the medium condition...Continue Reading

References

Mar 23, 2004·Nature Medicine·Lisette AcevedoWilliam C Sessa
Jul 6, 2004·Nature Medicine·Daniel J CeradiniGeoffrey C Gurtner
Aug 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Luciano C AmadoJoshua M Hare
Nov 24, 2006·Seminars in Reproductive Medicine·Pascale V GuillotNicholas M Fisk
Jun 15, 2007·Trends in Immunology·Isabelle PetitShahin Rafii
Nov 30, 2007·Pediatrics International : Official Journal of the Japan Pediatric Society·Takashi SasakiKazuo Shimizu
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Feb 2, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Faouzia ZemaniCatherine Boisson-Vidal
Nov 1, 2007·Stem Cell Research·Leo TimmersDominique P V de Kleijn
Feb 9, 2010·Stem Cell Research·Ruenn Chai LaiSai Kiang Lim
May 4, 2011·Stem Cells and Development·Sveva BolliniPaolo De Coppi
Jul 5, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dafni MoschidouPascale V Guillot
Jul 6, 2013·British Journal of Cancer·S KondoT Okusaka
Sep 7, 2013·Journal of Extracellular Vesicles·Chris GardinerIan L Sargent
Sep 7, 2013·Journal of Extracellular Vesicles·Jason Webber, Aled Clayton
Apr 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christina KlasenOmar El Bounkari
Oct 4, 2014·Journal of Extracellular Vesicles·Anita N BöingRienk Nieuwland
Mar 31, 2015·Journal of Extracellular Vesicles·Joanne Louise WeltonAled Clayton
Jul 22, 2015·Journal of Extracellular Vesicles·Richard J LobbAndreas Möller
Aug 13, 2015·Organic & Biomolecular Chemistry·Xabier OsteikoetxeaEdit Irén Buzás
Dec 25, 2015·Journal of Extracellular Vesicles·Romain LinaresAlain R Brisson
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joanna KowalClotilde Théry
Nov 1, 2016·Cardiovascular Drugs and Therapy·Sean M DavidsonDerek M Yellon
Mar 1, 2017·Nature Methods·UNKNOWN EV-TRACK ConsortiumAn Hendrix
Apr 4, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Emma A MolPieter Vader
Apr 16, 2017·Circulation·Geoffrey de CoutoEduardo Marbán
May 13, 2017·Circulation Research·Dilyana TodorovaFrançoise Dignat-George
Oct 19, 2017·Cardiovascular Research·Oliver J ZiffSean M Davidson
Nov 11, 2017·Molecular Aspects of Medicine·Sean M Davidson, Derek M Yellon
Dec 1, 2017·Journal of Extracellular Vesicles·Kaloyan TakovSean M Davidson
Jan 18, 2018·Nature Reviews. Molecular Cell Biology·Guillaume van NielGraça Raposo
Jan 20, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Merry L LindseyGerd Heusch

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Citations

Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Edoardo MaghinSveva Bollini
Jul 1, 2020·BioMed Research International·Dong GuoMingming Zhang
Sep 10, 2020·International Journal of Molecular Sciences·Karim SidhomAyesha Saleem
Jan 23, 2021·International Journal of Molecular Sciences·Quan-Wen LiuHong-Bo Xin
Dec 31, 2020·International Journal of Molecular Sciences·Yi-Hsien FangYen-Wen Liu
Dec 12, 2020·Advanced Science·Britta F HettichJean-Christophe Leroux
Mar 25, 2020·International Journal of Molecular Sciences·Danilo BucaPaola Lanuti
Feb 20, 2021·Frontiers in Pharmacology·Nicolas Herrera-ZeladaJaime A Riquelme
Apr 3, 2021·Journal of Cellular and Molecular Medicine·Miroslava KatsurSean M Davidson
Jun 6, 2021·Basic Research in Cardiology·Yen-Nien LinEugenio Cingolani
Jul 9, 2021·Frontiers in Cell and Developmental Biology·Yan ZhangGuokun Zhang
Jul 27, 2021·Frontiers in Cardiovascular Medicine·Zhu Yuan, Weiqiang Huang
Sep 18, 2021·Frontiers in Cell and Developmental Biology·Rafael Sánchez-SánchezPilar Sepúlveda

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Methods Mentioned

BETA
size-exclusion chromatography
flow cytometry
protein assay
Assay
electron microscopy
Profiler
biopsies

Software Mentioned

STRING
GraphPad Prism
Proteome Profiler
EV
GraphPad
Proteome Discoverer
Profiler
SequestHT
TRACK
NTA

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