Small G proteins are expressed ubiquitously in lymphoid cells and do not correspond to Bcl-2

Nature
K MonicaM L Cleary

Abstract

The bcl-2 gene is consistently associated with t(14; 18) chromosomal translocations observed in a large fraction of human B-cell lymphomas. The t(14; 18) translocation results in deregulated expression of the bcl-2 gene and synthesis of inappropriately high levels of the Bcl-2 protein. Gene transfer studies suggest a role for Bcl-2 in cell survival, growth enhancement and oncogenic transformation. To test the suggestion that GTP-binding by Bcl-2 may mediate its biological effects we characterized the GTP-binding proteins in lymphoid cells expressing Bcl-2. Expression of several small GTP-binding proteins was found to be ubiquitous and did not vary with levels of Bcl-2. By using immunological, electrophoretic and cell-fractionation techniques, we separated Bcl-2 from G proteins of small relative molecular mass (Mr) and showed that it is incapable of binding GTP. Our results show that small Mr G proteins are widely expressed in lymphoid cells and that Bcl-2 is not a novel member of this GTP-binding protein family.

References

Nov 9, 1989·Nature·S HaldarC M Croce
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y Tsujimoto
Nov 17, 1988·Nature·J C ReedP C Nowell
Jul 1, 1986·The Journal of Experimental Medicine·M L ClearyJ Sklar
Jan 1, 1986·Annual Review of Cell Biology·L Stryer, H R Bourne
Jan 18, 1988·FEBS Letters·G M Bokoch, C A Parkos
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E G Lapetina, B R Reep
Nov 5, 1987·The New England Journal of Medicine·L M WeissM L Cleary
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E A NiggS J Singer
Nov 11, 1982·The New England Journal of Medicine·J J YunisA Theologides

❮ Previous
Next ❯

Citations

Feb 15, 1996·European Journal of Biochemistry·A J HaleG T Williams
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M Brada
Sep 30, 1999·Experimental Cell Research·S NaderiH K Blomhoff
Nov 18, 1993·Nature·M J Fernandez-Sarabia, J R Bischoff
Oct 21, 1992·International Journal of Cancer. Journal International Du Cancer·A E MilnerC D Gregory
Dec 1, 1991·Molecular and Cellular Biology·K MonicaM L Cleary

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.