Small GTPase RhoA and its effector rho kinase mediate oxygen glucose deprivation-evoked in vitro cerebral barrier dysfunction

Stroke; a Journal of Cerebral Circulation
Claire AllenUlvi Bayraktutan

Abstract

Enhanced vascular permeability attributable to disruption of blood-brain barrier results in the development of cerebral edema after stroke. Using an in vitro model of the brain barrier composed of human brain microvascular endothelial cells and human astrocytes, this study explored whether small GTPase RhoA and its effector protein Rho kinase were involved in permeability changes mediated by oxygen-glucose deprivation (OGD), key pathological phenomena during ischemic stroke. OGD increased RhoA and Rho kinase protein expressions in human brain microvascular endothelial cells and human astrocytes while increasing or unaffecting that of endothelial nitric oxide synthase in respective cells. Reperfusion attenuated the expression and activity of RhoA and Rho kinase in both cell types compared to their counterparts exposed to equal periods of OGD alone while selectively increasing human brain microvascular endothelial cells endothelial nitric oxide synthase protein levels. OGD compromised the barrier integrity as confirmed by decreases in transendothelial electric resistance and concomitant increases in flux of permeability markers sodium fluorescein and Evan's blue albumin across cocultures. Transfection of cells with constitutively...Continue Reading

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Citations

Jun 8, 2014·Molecular and Cellular Neurosciences·Zuraidah Abdullah, Ulvi Bayraktutan
Aug 22, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kirtiman SrivastavaUlvi Bayraktutan
Jun 5, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Martha L Pinzón-DazaChiara Riganti
Nov 4, 2015·Biochimica Et Biophysica Acta·Kamini Rakkar, Ulvi Bayraktutan
Mar 3, 2015·International Journal of Stroke : Official Journal of the International Stroke Society·Philip M Bath, Joanna M Wardlaw
Apr 27, 2013·Diabetes, Obesity & Metabolism·B Shao, U Bayraktutan
Nov 8, 2015·Molecular and Cellular Neurosciences·Zuraidah AbdullahUlvi Bayraktutan
Aug 29, 2016·Journal of Alzheimer's Disease : JAD·Murali Vijayan, P Hemachandra Reddy
Feb 6, 2017·Circulation Research·Xiaoming HuFrank M Faraci
Dec 15, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Susanna Camós, Judith Mallolas
Oct 20, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gina HadleyAlastair M Buchan
Sep 1, 2020·Cellular and Molecular Neurobiology·Ziyu WangWeirong Fang
Jan 1, 2016·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·R G EsinI Kh Khairullin
Jan 9, 2019·Frontiers in Neuroscience·Peter Van Dyken, Baptiste Lacoste
Aug 28, 2020·Frontiers in Physiology·Moises Freitas-AndradeBaptiste Lacoste

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