Small interfering RNA-mediated silencing of heat shock protein 27 (HSP27) Increases chemosensitivity to paclitaxel by increasing production of reactive oxygen species in human ovarian cancer cells (HO8910)

The Journal of International Medical Research
T F SongPeiLing Li

Abstract

Increasing evidence indicates that reactive oxygen species (ROS) are involved in paclitaxel cytotoxicity. Modulating the oxidant-antioxidant status of tumour cells may increase the antitumour activity of paclitaxel. The cytoprotective roles of heat shock protein 27 (HSP27) include chaperoning cellular proteins, regulating apoptotic signalling and modulating oxidative stress. Immunohistochemical staining for HSP27 in human ovarian cancer specimens showed HSP27 was associated with aggressive malignant ovarian disease. Small interfering RNA (siRNA) was used to down-regulate HSP27 in human ovarian cancer cells (HO8910). Reduction of HSP27 expression increased the in vitro chemosensitivity of HO8910 cells to paclitaxel and increased paclitaxel-induced apoptosis and ROS production, although the ROS scavenger, N-acetyl-L-cysteine, partly offset the effects of HSP27 siRNA. Thus, gene knock-down of HSP27 offsets the role of this protein in resisting oxidant stress, thereby indirectly increasing the sensitivity of cells to paclitaxel. The siRNA-induced knock-down of HSP27 could be a novel and potent strategy to help overcome chemotherapeutic resistance to paclitaxel in epithelial ovarian cancer cells.

References

Jan 1, 1995·Pharmacology & Therapeutics·G PowisJ Oblong
Jul 31, 1999·The Journal of Biological Chemistry·C ScaffidiM E Peter
Feb 3, 2000·Nature Cell Biology·F Wianny, M Zernicka-Goetz
Oct 21, 2000·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·J L BenedetS Pecorelli
Jan 31, 2002·Annual Review of Medicine·Michael M Gottesman
Apr 26, 2002·Cell Death and Differentiation·C Garrido
Oct 25, 2003·Oncogene·George A OrrSusan Band Horwitz
Aug 12, 2004·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Y TanakaI Kohno
Sep 29, 2004·The Journal of Biological Chemistry·Soo-Jung ParkAhmad R Safa
Jul 26, 2005·Cell Stress & Chaperones·Daniel R Ciocca, Stuart K Calderwood
Sep 17, 2005·Cancer Research·Balakrishnan RamanathanYeong-Shiau Pu
Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·S HonoreD Braguer
Feb 2, 2006·International Journal of Cancer. Journal International Du Cancer·Jérôme AlexandreFrançois Goldwasser
May 1, 2007·FEBS Letters·André-Patrick ArrigoPatrick Vicart
May 31, 2007·Journal of Biosciences·Richa AryaSubhash C Lakhotia

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Citations

Jun 19, 2013·Journal of Medicinal Chemistry·Bo ZhongBin Su
Oct 27, 2011·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Huiling LiuBingdong Zhu
Jan 26, 2016·International Journal of Molecular Sciences·Mitsuru OkunoIchiro Yasuda
Nov 17, 2010·Cancer Letters·Gaëtan JegoCarmen Garrido
Sep 1, 2015·Critical Reviews in Oncology/hematology·M B StopeA Mustea
Sep 3, 2013·International Journal of Cancer. Journal International Du Cancer·Simona PavanMaria Flavia Di Renzo
Aug 2, 2000·American Journal of Obstetrics and Gynecology·I KorneevaS S Witkin
Mar 10, 2018·Scientific Reports·Jee-Youn KimJaeho Cho
May 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·In-Hyoung YangSung-Dae Cho
Dec 30, 2017·Oncotarget·Byeol ChoiYun-Sil Lee
Jan 4, 2020·Oncology Letters·Warne Pedro De AndradeAgnaldo Lopes Da Silva Filho
Dec 9, 2020·International Journal of Molecular Sciences·Kathleen Wantoch von RekowskiGerd Bendas
Jul 18, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Bingwei ZhangKe Tan

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