Small interfering RNA therapy against carbohydrate sulfotransferase 15 inhibits cardiac remodeling in rats with dilated cardiomyopathy

Cellular Signalling
Kenichi WatanabeKenji Suzuki

Abstract

Carbohydrate sulfotransferase 15 (CHST15) is a sulfotransferase responsible for biosynthesis of chondroitin sulfate E (CS-E), which plays important roles in numerous biological events such as biosynthesis of proinflammatory cytokines. However, the effects of CHST15 siRNA in rats with chronic heart failure (CHF) after experimental autoimmune myocarditis (EAM) have not yet been investigated. CHF was elicited in Lewis rats by immunization with cardiac myosin, and after immunization, the rats were divided into two groups and treated with either CHST15 siRNA (2μg/week) or vehicle. Age matched normal rats without immunizations were also included in this study. After 7weeks of treatment, we investigated the effects of CHST15 siRNA on cardiac function, proinflammatory cytokines, and cardiac remodeling in EAM rats. Myocardial functional parameters measured by hemodynamic and echocardiographic studies were significantly improved by CHST15 siRNA treatment in rats with CHF compared with that of vehicle-treated CHF rats. CHST15 siRNA significantly reduced cardiac fibrosis, and hypertrophy and its marker molecules (left ventricular (LV) mRNA expressions of transforming growth factor beta1, collagens I and III, and atrial natriuretic peptide)...Continue Reading

References

Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R EliakimE Razin
Aug 1, 1989·Biomaterials·A K GargH G Garg
Nov 1, 1985·Journal of the American College of Cardiology·C P TaliercioA J Tajik
Sep 1, 1966·Journal of Biochemistry·Y KawaiK Anno
Apr 14, 1995·The Journal of Biological Chemistry·E SchönherrH Kresse
Dec 1, 1994·British Heart Journal·A MatsumoriS Sasayama
Aug 1, 1994·Circulation Research·M KodamaA Shibata
Sep 1, 1993·Matrix : Collagen and Related Research·R I BasheyR Heimer
Mar 1, 1993·Cardiovascular Research·K T WeberJ S Janicki
Oct 1, 1995·Journal of Molecular and Cellular Cardiology·R HeimerS A Jimenez
Feb 10, 1997·The Journal of Cell Biology·K G DanielsonR V Iozzo
Oct 6, 1998·Annual Review of Biochemistry·R V Iozzo
Jan 30, 1999·Genes & Development·P A Sharp
May 24, 2000·The Journal of Biological Chemistry·D R KeeneR V Iozzo
Aug 6, 2000·British Journal of Pharmacology·K WatanabeY Aizawa
Aug 16, 2002·Connective Tissue Research·Denis M MedeirosSylvia A McCune
Oct 9, 2002·Medicinal Research Reviews·Koichi Honke, Naoyuki Taniguchi
Jul 11, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ayman Al Haj ZenBruno Gogly
Oct 22, 2003·Current Opinion in Structural Biology·Marion Kusche-Gullberg, Lena Kjellén
Nov 5, 2004·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Luiz Menezes FalcãoPeter Adriaan van Zwieten
Nov 19, 2004·European Journal of Immunology·Suresh S PalaniyandiYoshifusa Aizawa
Nov 8, 2005·Biological & Pharmaceutical Bulletin·Suresh Palaniyandi SelvarajYoshifusa Aizawa
Feb 4, 2006·The Journal of Allergy and Clinical Immunology·Calman Prussin, Dean D Metcalfe
Oct 19, 2006·Journal of the American College of Cardiology·Andreas S BarthHolger Sültmann

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Citations

Apr 10, 2016·International Journal of Biological Macromolecules·Hui TaoKai-Hu Shi
Aug 10, 2017·Applied Microbiology and Biotechnology·Chit TamTzi Bun Ng
Oct 29, 2020·European Journal of Human Genetics : EJHG·Sanni E RuotsalainenJukka Koskela

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