Small Intestinal Permeability and Gut-Transit Time Determined with Low and High Molecular Weight Fluorescein Isothiocyanate-Dextrans in C3H Mice

Nutrients
Anni Woting, Michael Blaut

Abstract

Protocols for intestinal permeability measurements in mice using 4-kDa fluorescein isothiocyanate-conjugated (FITC) dextran differ considerably among laboratories on the blood-sampling time. To find the optimal point in time for blood sampling, we administered 4-kDa FITC dextran to C3H mice and monitored the marker in plasma over 8 h. We also determined gut-transit time using 70-kDa FITC dextran, which does not cross the intestinal epithelium. The 4-kDa FITC dextran concentration in plasma reached its maximum 45 min after administration. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability.

References

Sep 11, 2007·Biological & Pharmaceutical Bulletin·Koyo NishidaToshiyuki Sakaeda
Dec 14, 2011·The American Journal of Pathology·Ravi M PatelPatricia W Lin
Feb 6, 2013·Gastroenterology·Purna C KashyapJustin L Sonnenburg
Nov 20, 2014·BMC Gastroenterology·Stephan C BischoffJerry M Wells
Jan 18, 2015·Journal of Immunological Methods·Lirui WangBernd Schnabl
Nov 2, 2015·Digestive Diseases and Sciences·Valentina VolynetsStephan C Bischoff
Oct 21, 2016·Clinical and Translational Gastroenterology·Julia KönigRobert-Jan Brummer
Jan 10, 2017·Cellular and Molecular Gastroenterology and Hepatology·Vincenza CifarelliNada A Abumrad
Mar 23, 2017·Lab Animal·Craig L Franklin, Aaron C Ericsson
Feb 2, 2018·Digestive Diseases and Sciences·Xubiao NieBiguang Tuo

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Citations

Jan 7, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Atreju I LackeyJudith Storch
Jun 14, 2020·The Journal of Experimental Biology·Kaylen Brzezinski, Heath A MacMillan
Apr 11, 2020·Mediators of Inflammation·Zhou ShangxunHuang Yang
Oct 13, 2020·Frontiers in Bioengineering and Biotechnology·Maria Helena MacedoBruno Sarmento
Nov 26, 2020·Cellular and Molecular Gastroenterology and Hepatology·John P KennellyRené L Jacobs
Mar 16, 2021·Frontiers in Endocrinology·Jenna Elizabeth HuntHannelouise Kissow
Apr 1, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Cláudia AzevedoBruno Sarmento
Apr 9, 2021·Scientific Reports·Mohamed Karim MaghrabyRobert H J Bandsma
May 6, 2021·EMBO Molecular Medicine·Jonathan Trujillo-VieraGrzegorz Sumara
Mar 31, 2021·Acta Physiologica·Elke M MuntjewerffSushil K Mahata
Jun 5, 2021·Cancer Research·David R Soto-PantojaKatherine L Cook
Nov 4, 2021·Cellular and Molecular Life Sciences : CMLS·Hanna Ilchmann-DiounouSandrine Menard
Oct 25, 2021·Gastroenterology·Anthony J TreichelArthur Beyder
Jan 1, 2022·Proceedings of the National Academy of Sciences of the United States of America·Shreya DasWei Sun

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