Small intestinal submucosa-derived extracellular matrix bioscaffold significantly enhances angiogenic factor secretion from human mesenchymal stromal cells

Stem Cell Research & Therapy
Xin LinClaus Svane Sondergaard

Abstract

The in vivo therapeutic effect of mesenchymal stromal cells (MSCs) is currently believed to be tightly linked to their paracrine secretion ability. However, insufficient or imprecise cell delivery, low cell survival and retention post-transplant, along with harsh donor site microenvironments, are major barriers to the clinical success of MSC therapies. Here we tested a small intestinal submucosa (SIS)-derived extracellular matrix (ECM) bioscaffold augmented with MSCs, with the hypothesis that they will facilitate the precise delivery of increased numbers of MSCs therefore improving cell viability and retention. In this study, we evaluated the secretion of angiogenic factors from three human MSC lines cultured on SIS ECM. We used human antibody array and enzyme-linked immunosorbent assay to measure the level of angiogenic factors released from MSCs when cultured on SIS ECM or regular tissue culture plastic. We tested MSCs cultured for three different time points. We found that the SIS ECM culture environment can significantly enhance the release of several angiogenic factors when compared to MSCs cultured on standard tissue culture plastic. Specifically, vascular endothelial growth factor and interleukin-8 secretion was signific...Continue Reading

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Citations

Aug 24, 2016·Drug Delivery and Translational Research·Matthias SchweinlinFlorian Groeber
Feb 15, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marek KaweckiAleksandra Kasperczyk
Nov 24, 2018·Journal of Biomedical Materials Research. Part a·Yanhui JiXiaodong Guo
Aug 23, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guangxiu CaoXiaoming Li
Mar 13, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Xin ShiPing Zhang
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Chunqing MengTingfang Sun
Jan 1, 2020·Progress in Biomedical Engineering·Guang YangJohn P Fisher
Aug 14, 2017·ACS Biomaterials Science & Engineering·Qi XingFeng Zhao

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Methods Mentioned

BETA
antibody array
ELISA
flow cytometry

Software Mentioned

ImageJ Angiogenesis Analyzer
LI
COR image studio lite
Image J

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