Abstract
Oxidative stress and ageing are important factors contributing to the pathogenesis of Alzheimer's disease (AD), which is associated with neuronal damage and β-amyloid (Aβ) deposition. The p66shc adaptor protein is important for the regulation of oxidative stress and ageing. In the present study, SH-SY5Y human neuroblastoma cells were treated with anisomycin in order to establish a cell model of oxidative stress-induced neuronal damage. The results from quantitative polymerase chain reaction, enzyme-linked immunosorbent assay and western blotting demonstrated that anisomycin was able to stimulate the secretion of Aβ1-42 from SH-SY5Y cells and upregulate the expression levels of p66shc, which was associated with concomitant damage to SH-SY5Y cells. In addition, the protective effects of various small molecule compounds with antioxidant properties against neuronal damage were evaluated. Notably, treatment of SH-SY5Y cells with gallic acid was associated with significant downregulation of p66shc protein expression levels, reduced anisomycin-induced secretion of Aβ1-42, and increased superoxide dismutase activity and acetylcholine secretion levels. The results of the present study suggested that anisomycin is able to promote oxidati...Continue Reading
References
Dec 2, 1999·Nature·E MigliaccioP G Pelicci
Jul 20, 2002·Science·John Hardy, Dennis J Selkoe
Aug 6, 2004·Nature·Mark P Mattson
Jul 1, 2006·Experimental Gerontology·Alessandra BerryFrancesca Cirulli
May 12, 2007·Brain Pathology·Jürgen GötzLars M Ittner
Dec 11, 2007·Experimental Gerontology·A BerryF Cirulli
Dec 15, 2010·Science·Kwasi G MawuenyegaRandall J Bateman
Aug 17, 2011·Neuroscience·X GuoL Li
Dec 2, 2011·Archives of Gerontology and Geriatrics·Kiyoaki SoneNozomu Mori
Nov 9, 2012·Molecular Medicine Reports·Gang ZouTe Liu
Mar 26, 2013·Neuroscience and Biobehavioral Reviews·Alessandra Berry, Francesca Cirulli
Nov 14, 2013·Experimental and Therapeutic Medicine·Qing ChenTe Liu
Dec 3, 2014·Brain Research Bulletin·R R PenaG S Pereira
Jan 13, 2015·Neuropharmacology·Barbara A SorgLynn Churchill
Mar 31, 2015·Aging and Disease·Chiara PriamiEnrica Migliaccio
Apr 4, 2015·Cell Death & Disease·X LiZ Ma
Apr 19, 2015·Molecular Cancer·Sahar S BhatFirdous A Khanday
Jul 24, 2015·Cell Death & Disease·W ShanJ Yao
Aug 8, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jonathan D DubueClayton T Dickson
Citations
Aug 31, 2017·Scientific Reports·Shi-Jie ZhangQi Wang
Sep 13, 2018·Reviews in the Neurosciences·Xiao Juan SuDezhi Mu
Aug 17, 2020·Ageing Research Reviews·Hilal Ahmad MirFirdous A Khanday
Sep 19, 2021·Human Cell·Jian WangJianhong Wang