Small molecule MALDI MS imaging: Current technologies and future challenges

Methods : a Companion to Methods in Enzymology
Paul J Trim, Marten F Snel

Abstract

Imaging of specific small molecules is particularly challenging using conventional optical microscopy techniques. This has led to the development of alternative imaging modalities, including mass spectrometry (MS)-based methods. This review aims to provide an overview of the technologies, methods and future directions of laser-based mass spectrometry imaging (MSI) of small molecules. In particular it will focus on matrix-assisted laser desorption/ionization (MALDI) as the ion source, although other laser mass spectrometry methods will also be discussed to provide context, both historical and current. Small molecule MALDI MSI has been performed on a wide variety of instrument platforms: these are reviewed, as are the laser systems that are commonly used in this technique. Instrumentation and methodology cross over in the areas of achieving optimal spatial resolution, a key parameter in obtaining meaningful data. Also discussed is sample preparation, which is pivotal in maintaining sample integrity, providing a true reflection of the distribution of analytes, spatial resolution and sensitivity. Like all developing analytical techniques there are challenges to be overcome. Two of these are dealing with sample complexity and obtain...Continue Reading

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Jun 22, 2016·Methods : a Companion to Methods in Enzymology·Rainer Cramer
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Aug 26, 2020·Journal of the American Society for Mass Spectrometry·Nolan McLaughlinKatherine A Stumpo
Sep 5, 2020·Journal of the American Society for Mass Spectrometry·Elizabeth K NeumannJeffrey M Spraggins

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