Small molecule-protein interactions in branch migration thermodynamics: modelling and application in the homogeneous detection of proteins and small molecules

The Analyst
Xing ZhouXianjin Xiao

Abstract

We have disclosed the unique inhibition effect of small molecule-protein interactions toward the DNA branch migration process and constructed a complete thermodynamic model for it. The disclosed effect was further coupled with the steric hindrance effect to establish a homogeneous assay for proteins and small molecules with ultra-high inhibition factors and sensitivity.

References

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Citations

Jul 4, 2020·Chemical Communications : Chem Comm·Longjie LiXianjin Xiao

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
DNA probe assay
Fluorescence

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