Small-molecule reagents for cellular pull-down experiments

Bioconjugate Chemistry
Xiang WangStuart L Schreiber

Abstract

Affinity purification of interacting proteins from cellular extracts is a powerful technique for identifying the cellular targets of small molecules. Affinity matrix-based small-molecule reagents are usually prepared by conjugating small molecules of interest to a solid support such as agarose. This protocol describes an efficient and robust method to immobilize small molecules containing a primary alcohol, a common functional group in small molecules, especially in small molecules prepared using diversity-oriented synthesis. This method comprises one element of a systematic approach to the target identification problem in chemical biology.

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Citations

Dec 24, 2009·Bioconjugate Chemistry·Carlos TassaStanley Y Shaw
Sep 6, 2011·Journal of the American Chemical Society·Rebecca L SimmonsAndrew G Myers
Aug 14, 2009·Journal of the American Chemical Society·Chambers C HughesWilliam Fenical
Mar 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shao-En OngSteven A Carr
Oct 15, 2013·Chembiochem : a European Journal of Chemical Biology·Katherine ZlotkowskiDionicio Siegel
Dec 27, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jenny M TamJatin M Vyas
Dec 15, 2011·British Journal of Cancer·G Roti, K Stegmaier
Dec 4, 2008·Journal of Combinatorial Chemistry·Troy D RybaLisa A Marcaurelle

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