Abstract
As dormant phenotypic variants of bacteria, persisters account for many chronic infections affecting human health. Despite numerous studies, the role of small non-coding RNA (sRNA) in bacterial persistence has not been reported. To investigate the role of Hfq-interacting sRNA in persistence, we constructed the deletion mutants of 20 Hfq-interacting sRNAs (RyhB, GcvB, MgrR, RybB, MicF, SgrS, RprA, DicF, SsrS, FnrS, GadY, DsrA, OmrB, ArcZ, RyeB, RydC, OmrA, MicA, MicC, and ChiX) to assess their persistence capacity in uropathogenic Escherichia coli strain UTI89 and identified a new sRNA RyhB being involved in persister formation. The ryhB-knockout mutant had significant defect in persistence to a diverse range of antibiotics (levofloxacin, cefotaxime, gentamicin) and stresses (hyperosmosis, acid, and heat) in both exponential phase and stationary phase. In addition, the effect of RyhB on persistence was synergistic with ppGpp and Fur protein. RNA-Seq analysis indicated that the ryhB-knockout mutant had a hyperactive metabolic state compared with the parent strain. Interestingly, increased adenosine triphosphate (ATP) levels and altered NAD+/NADH ratios were observed in the ryhB-knockout mutant. Our findings represent a new level ...Continue Reading
References
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric Massé, Susan Gottesman
May 15, 2002·Cell·Karen M Wassarman
Nov 19, 2003·Molecular Microbiology·Shaleen B KorchThomas M Hill
Jan 24, 2004·The EMBO Journal·Thomas A Geissmann, Danièle Touati
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Catherine VilchèzeWilliam R Jacobs
Nov 29, 2005·Comparative Immunology, Microbiology and Infectious Diseases·G RamaS Sharma
Dec 19, 2006·Journal of Bacteriology·Julie E ButtonNatividad Ruiz
Apr 11, 2007·Antimicrobial Agents and Chemotherapy·Yongfang Li, Ying Zhang
May 7, 2008·Infection and Immunity·Richard R KulesusMatthew A Mulvey
Nov 17, 2009·Biochemical and Biophysical Research Communications·Younghoon Kim, Thomas K Wood
Jan 1, 2010·FEMS Microbiology Letters·Chao MaYing Zhang
Jan 19, 2010·Current Opinion in Microbiology·Yanjie Chao, Jörg Vogel
Mar 17, 2010·Antimicrobial Agents and Chemotherapy·Matthew G Blango, Matthew A Mulvey
Jun 10, 2010·Annual Review of Microbiology·Kim Lewis
Jun 10, 2010·PLoS Pathogens·Svetlana ChabelskayaBrice Felden
Jul 20, 2010·Cell Host & Microbe·Kai Papenfort, Jörg Vogel
Aug 5, 2010·The EMBO Journal·Pierre Mandin, Susan Gottesman
Oct 29, 2010·Cold Spring Harbor Perspectives in Biology·Susan Gottesman, Gisela Storz
May 13, 2011·Nature·Kyle R AllisonJames J Collins
May 24, 2011·Journal of Bacteriology·Hannes LuidaleppTanel Tenson
Sep 23, 2011·Current Opinion in Microbiology·Kyle R AllisonJames J Collins
Dec 27, 2011·Nature Structural & Molecular Biology·Nour SayedBrice Felden
Mar 7, 2012·Antimicrobial Agents and Chemotherapy·Ying ZhangMichael R Barer
Mar 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Liron ArgamanShoshy Altuvia
May 2, 2012·RNA Biology·Magali HébrardJay C D Hinton
Sep 3, 2013·Cell·Etienne MaisonneuveKenn Gerdes
Aug 5, 2014·Current Opinion in Microbiology·Bruce R LevinKlas I Udekwu
Sep 24, 2014·Infection and Immunity·Gaëlle PorcheronCharles M Dozois
Mar 1, 2015·The Journal of Antimicrobial Chemotherapy·Taeyeon KimKwang-Sun Kim
Jan 1, 2014·Emerging Microbes & Infections·Ying Zhang
Jan 19, 2016·Frontiers in Microbiology·Wenjie WangYing Zhang
Feb 26, 2016·Current Opinion in Microbiology·Taylor B UpdegroveGisela Storz
Jul 12, 2016·Nature Microbiology·Brian P ConlonKim Lewis
Aug 31, 2016·Nature Microbiology·Brian P ConlonKim Lewis
Oct 19, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Shu-Cheng LiuZi-Li Pang
Nov 9, 2016·Frontiers in Microbiology·Tao XuYing Zhang
Dec 17, 2016·Science·Alexander HarmsKenn Gerdes
Dec 23, 2016·Journal of Bioscience and Bioengineering·Soya MaedaAtsushi Yokota
Feb 9, 2017·MBio·Yue ShanKim Lewis
Nov 3, 2017·Biotechnology for Biofuels·Yan WuYuan Lu
Citations
Jul 23, 2019·Nucleic Acids Research·Todd A CameronNicholas R De Lay
Jun 2, 2020·Future Microbiology·Mariana Am FerreiraKênia V Dos Santos
Nov 30, 2018·International Journal of Molecular Sciences·Tiago PitaJorge H Leitão
Feb 12, 2021·International Journal of Molecular Sciences·Nicole RaadNorbert Polacek
Feb 13, 2021·Microbiological Research·Yuanyuan XuWenhong Zhang
Sep 22, 2020·Trends in Genetics : TIG·Daniel G MediatiJai J Tree
Apr 4, 2021·Microorganisms·Diego PeñalozaIván L Calderón
Jun 23, 2021·Molecular Cell·Tom DendoovenKatarzyna J Bandyra
Jul 3, 2021·Microorganisms·Iván L CalderónLillian G Acuña